{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:16:54Z","timestamp":1789154214660,"version":"build-2803163510"},"reference-count":74,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:00:00Z","timestamp":1725840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006565","name":"University of Johannesburg","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006565","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Asparagus<\/jats:italic> species are naturally distributed worldwide and are known for their pharmacological properties that offer cures for various ailments. However, the metabolic choreography of these <jats:italic>Asparagus<\/jats:italic> species is not well characterized, and the compounds contributing to their bioactivities remain unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to profile and compare the metabolomes of three <jats:italic>Asparagus<\/jats:italic> species cladodes using different solvent extractions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An ultra\u2010high performance liquid chromatography\u2013quadrupole time\u2010of\u2010flight mass spectrometry\u2010based metabolomics and molecular networking approach was used to study the effects of different solvents (ethyl acetate, methanol, and chloroform) with varying polarity on metabolites extraction and identification of bioactive compounds from three <jats:italic>Asparagus<\/jats:italic> species cladodes (<jats:styled-content style=\"fixed-case\"><jats:italic>Asparagus falcatus<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Asparagus plumosus<\/jats:italic><\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\"><jats:italic>Asparagus densiflorus<\/jats:italic><\/jats:styled-content> \u2018Meyersii\u2019).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Multivariate statistical analyses (mainly principal component analysis) revealed a significant separation between the three solvents and the three species, indicating notable metabolic differences. A total of 118 metabolites were identified in the three species extracted with the different solvents, with methanolic and chloroform extracts containing more metabolites compared with ethyl acetate extracts. These metabolites were identified as belonging to the flavonoids, cinnamic acids, organooxygen compounds, steroids, fatty acids, benzenes, and glycerophospholipids compound classes. Furthermore, these compounds classes were differentially distributed among the three species, indicating chemical\/chemotaxis differences between the compared species. Chloroform and methanol are recommended as the optimal solvents to obtain a high content of phytochemical compounds from <jats:italic>Asparagus<\/jats:italic> species cladodes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3446","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T02:19:18Z","timestamp":1725934758000},"page":"506-519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparative metabolites profiling of different solvent extracts of <scp><i>Asparagus<\/i><\/scp> species cladodes using liquid chromatography\u2013mass spectrometry\u2010based metabolomics and molecular networking"],"prefix":"10.1002","volume":"36","author":[{"given":"Pfano W.","family":"Maphari","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Science University of Johannesburg  Auckland Park Gauteng South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mthokozisi B. 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