{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:23:20Z","timestamp":1777397000159,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Reactive oxygen species (ROS) and ultraviolet (UV) light are significant factors to impair skin disorders. <jats:styled-content style=\"fixed-case\"><jats:italic>Impatiens balsamina<\/jats:italic><\/jats:styled-content> Linn. (IB), a traditional Chinese and Thai herbal medicine, has long been used to treat skin and nail diseases, potentially due to its radical\u2010scavenging properties. However, specific antioxidant compounds in IB have not been well defined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This work aims to rapidly identify, target, and isolate antioxidant biomarkers in IB using at\u2010line LC\u2010ESI\u2010QTOF\u2010(MS\/MS) coupled with a simple DPPH assay and comprehensively investigate the antioxidant activities of IB extract and isolated biomarker.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Following liquid chromatography (LC), the eluent of IB extract was split into two streams (9:1 ratio). The majority was fractionated for DPPH assay in 96\u2010well plates, whereas 10% underwent chemical identification using ESI\u2010QTOF\u2010MS. Antioxidants in IB were identified, targeted, and promptly isolated through transfer from analytical LC to preparative HPLC. IB and the isolated biomarkers were evaluated for antioxidant effects using various antiradical assays and in suppressing ROS induced by UV in skin cells, keratinocytes, and fibroblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirty\u2010one chemical constituents were identified, with four tentatively identified as potent antioxidants. Kaempferol emerged as a potential antioxidant biomarker in IB, exhibiting superior antioxidant activity in various in\u00a0vitro assays compared with positive controls. Both IB extract and kaempferol effectively reduced UVB\u2010induced ROS in skin cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study represents the first comprehensive identification of antioxidants and chemical constituents in IB, pinpointing kaempferol as a key antioxidant biomarker. Its rapid identification using at\u2010line techniques holds promise for advancing bioactive compound discovery in herbal medicine.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3461","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T22:39:55Z","timestamp":1730155195000},"page":"704-717","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["At\u2010Line LC\u2010QTOF\u2010ESI\u2010MS\/MS Fractionation of <scp><i>Impatiens balsamina<\/i><\/scp> Linn. 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