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Molecular docking confirms the inhibition of these compounds. The molecular interaction between \u03b1\u2010amylase and the active compound was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 101 compounds identified, 28 compounds had a strong inhibitory effect on <jats:italic>\u03b1<\/jats:italic>\u2010amylase. Molecular docking screening confirmed the inhibition of these compounds and evaluated the molecular interactions between <jats:italic>\u03b1<\/jats:italic>\u2010amylase and 30 active compounds, which strongly supported the experimental results. Among the evaluated compounds, onjisaponin R (<jats:bold>83<\/jats:bold>) and polygalaxanthone III (<jats:bold>11<\/jats:bold>) have the strongest inhibitory activity to <jats:italic>\u03b1<\/jats:italic>\u2010amylase (the binding energies were \u22129.639 and \u22128.972\u2009kcal\/mol, respectively) and are potential lead compounds against diabetes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study proved the feasibility of using the existing platform to screen the active ingredients in PR extract, and provided a practical method for the rapid screening of potential anti\u2010diabetic active ingredients in traditional Chinese medicine.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3465","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T06:34:24Z","timestamp":1731566064000},"page":"759-775","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Screening Inhibitors of <i>\u03b1<\/i>\u2010Amylase in <i>Polygala<\/i><i>Radix<\/i> Based on an Online Targeted Detection System and Molecular Docking"],"prefix":"10.1002","volume":"36","author":[{"given":"Zenghu","family":"Su","sequence":"first","affiliation":[{"name":"Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy Shaanxi University of Chinese Medicine  Xianyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9633-5566","authenticated-orcid":false,"given":"Hongbo","family":"Xu","sequence":"additional","affiliation":[{"name":"Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy Shaanxi University of Chinese Medicine  Xianyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shizhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy Shaanxi University of Chinese Medicine  Xianyang China"},{"name":"School of Pharmaceutical Sciences Peking University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuming","family":"Li","sequence":"additional","affiliation":[{"name":"Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy Shaanxi University of Chinese Medicine  Xianyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyu","family":"Weng","sequence":"additional","affiliation":[{"name":"Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy Shaanxi University of Chinese Medicine  Xianyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuangui","family":"Yang","sequence":"additional","affiliation":[{"name":"Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization, State Key Laboratory of Research &amp; Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Innovative Drug Research Center and College of Pharmacy Shaanxi University of Chinese Medicine  Xianyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.12.031"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1023713"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.08.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30058-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2021.462754"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4103\/ajm.ajm_53_20"},{"key":"e_1_2_8_8_1","unstructured":"C.JuanandS. 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