{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T21:52:51Z","timestamp":1787349171365,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Antimicrobial resistance and free radical\u2010mediated oxidative stress and inflammation involved in many pathological processes have become treatment challenges. One strategy is to search for antimicrobial and antioxidant ingredients from natural aromatic plants. This study established a rapid and high\u2010throughput effect\u2010component analysis method to screen active ingredients from <jats:styled-content style=\"fixed-case\"><jats:italic>Ligusticum chuanxiong<\/jats:italic><\/jats:styled-content> essential oil (CXEO).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The study aims to screen phthalides with antimicrobial and antioxidant activities from CXEO by high\u2010performance thin\u2010layer chromatography (HPTLC)\u2010bioautography combined with HPLC\u2010TOF\/MS method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Antimicrobial activity was evaluated by disc diffusion and micro broth dilution methods. Antioxidant capacity was performed by DPPH scavenging test. Phthalides in CXEO were identified using HPLC\u2010TOF\/MS method. HPTLC\u2013bioautography technique was established to screen phthalides of antifungal and antioxidant activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CXEO had significant inhibitory activity against <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans<\/jats:italic><\/jats:styled-content>, weak or undetected inhibitory activity against <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\"><jats:italic>Pseudomonas aeruginosa<\/jats:italic><\/jats:styled-content>. For tested strains of <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009albicans<\/jats:italic><\/jats:styled-content>, inhibition zone diameters ranged from 13 to 17\u2009mm, and MICs were from 0.5 to 2\u2009mg\u2009mL<jats:sup>\u22121<\/jats:sup>. CXEO also had strong antioxidant activity, IC<jats:sub>50<\/jats:sub> value for scavenging DPPH free radicals was 1.014\u2009\u00b1\u20090.014\u2009mg\u2009mL<jats:sup>\u22121<\/jats:sup>. Nine phthalides in CXEO were tentatively identified. Ligustilide and senkyunolide A were screened to have both antimicrobial activity against <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009albicans<\/jats:italic><\/jats:styled-content> and strong DPPH scavenging property.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HPTLC\u2010bioautography\u2013MS\u2010guided strategy is very practical for high\u2010throughput screening of antifungal and antioxidant phthalides from CXEO. In\u00a0vitro experiments have shown that phthalides and CXEO have good biological activities, which may be used to the treatment of <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009albicans<\/jats:italic><\/jats:styled-content> infection or oxidative stress damage caused by various diseases. The therapeutic potential should be validated in\u00a0vivo in the future.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pca.3498","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T19:05:27Z","timestamp":1736795127000},"page":"1130-1140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["HPTLC\u2010Bioautography\u2013MS\u2010Guided Strategy for the Detection of Phthalides With Antimicrobial and Antioxidant Activities From <scp><i>Ligusticum chuanxiong<\/i><\/scp> Essential Oil"],"prefix":"10.1002","volume":"36","author":[{"given":"Zhijun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Shanghai Changhai Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanghai Changhai Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Shanghai Eastern Hepatobiliary Surgery Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sicong","family":"Qiao","sequence":"additional","affiliation":[{"name":"School of Pharmacy East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengxin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanghai Changhai Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3347-5571","authenticated-orcid":false,"given":"Hongjie","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanghai Changhai Hospital Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,13]]},"reference":[{"key":"e_1_2_4_2_1","first-page":"42p","volume-title":"Pharmacopoeia of the People's Republic of China","author":"China Pharmacopoeia Committee","year":"2020"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2024.18.027"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X2050086X"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.19664\/j.cnki.1002\u20132392.230045"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2018.08.031"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.11920\/xnmdzk.2022.02.008"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20240221.601"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.877806"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1674\u20107860.2022.13.001"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.13192\/j.issn.1000\u20131719.2019.05.007"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.19378\/j.issn.1003\u20109783.2021.11.020"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2023.115540"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2018.02.050"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.4268\/cjcmm20162306"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27144589"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7751"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201045222\u2010y"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272\u2010001\u20101184\u20107"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01461"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117247"},{"issue":"2","key":"e_1_2_4_22_1","first-page":"16","article-title":"GC\u2010MS Analysis and Antioxidant Activity Study of Essential Oil From \n                     Ligusticum chuanxiong\n                   Hort","volume":"27","author":"Li S. 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