{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T14:21:55Z","timestamp":1780410115522,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Geum japonicum<\/jats:italic>\n                      Thunb. var.\n                      <jats:italic>chinense<\/jats:italic>\n                      F. Bolle (GJ) holds significance as a traditional Miao medicine, one of China's ethnic minorities, extensively utilized in the clinical management of cardiovascular ailments. However, little attention has been directed toward the chemical constituents of GJ, as well as the metabolic and distribution of its chemical constituents in\u00a0vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study is to qualitatively analyze the chemical constituents of GJ, as well as their prototype components and metabolites in\u00a0vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established a reliable method using ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole Exactive Orbitrap mass spectrometry\u00a0(UHPLC\u2010Q\u2010Exactive Orbitrap\u2010MS) to conduct a comprehensive analysis of GJ as well as systematically to screen the prototype and metabolite constituents in rat plasma, urine, feces, brain, heart, liver, spleen, and kidney after gastric perfusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 94 compounds were identified or tentatively characterized in GJ. Moreover, a total of 75 in\u00a0vivo components were identified from the plasma, urine, feces, brain, heart, liver, spleen, and kidney of rats gavaged with 500\u2009mg\/kg of GJ extract, including 19 prototype components and 56 metabolites. The metabolic pathways, including Phase I reactions and Phase II reactions, for the absorbed constituents, were explored and summarized. Moreover, the metabolic pathways of GJ were proposed, which shed light on their mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This is the first systematic study on the components of GJ and their metabolites in\u00a0vivo and in\u00a0vitro. The metabolite distribution and metabolic pathways of GJ in\u00a0vivo were further proposed.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3528","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T06:19:53Z","timestamp":1741933193000},"page":"1517-1538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Characterization and Identification of the Chemical Constituents and the Metabolites of\n                    <i>Geum japonicum<\/i>\n                    Thunb. var.\n                    <i>chinense<\/i>\n                    F. Bolle"],"prefix":"10.1002","volume":"36","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"},{"name":"First Affiliated Hospital Dalian Medical University  Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leilei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Beijing Changping District Food and Drug Safety Monitoring Center  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixia","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxu","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianxin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuju","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KaiYang","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0555-9191","authenticated-orcid":false,"given":"Liying","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"414","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Pharmacopoeia Commission of the People's Republic of China","year":"2023"},{"key":"e_1_2_6_3_1","first-page":"233","article-title":"Research Progress on Chemical Ingredients of Aleppo Avens and Their Pharmacological Effects","volume":"49","author":"Tao W.","year":"2018","journal-title":"Chinese herbal medicine"},{"key":"e_1_2_6_4_1","first-page":"89","article-title":"Quality Control of Aurantii Fructus in Yangyin Kouxiang Mixture","volume":"42","author":"Guo L. 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