{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T19:50:54Z","timestamp":1761162654503,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T00:00:00Z","timestamp":1743724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The SARS\u2010CoV\u20102 pandemic has revealed a deficiency in antiviral agents. Plants, traditionally used for respiratory infections, are valuable sources of antiviral compounds. Such a plant is the\n                      <jats:italic>Sideritis<\/jats:italic>\n                      L. taxa (mountain tea), traditionally used against cold and cough.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Accordingly, this study aimed to investigate the potential protective effects of dichloromethane extracts from\n                      <jats:italic>Sideritis<\/jats:italic>\n                      species against SARS\u2010CoV\u20102.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Eight\n                      <jats:italic>Sideritis<\/jats:italic>\n                      extracts were tested in an\n                      <jats:italic>in vitro<\/jats:italic>\n                      pretreatment assay to assess the protective effect against SARS\u2010CoV\u20102. Therefore, infectious virus particles were pre\u2010incubated with the extract, then incubated with Vero E6 cells to finally measure cell viability as a surrogate for virus infection. Untargeted analyses (GC\u2013MS and LC\u2010PDA\u2010HRESIMS) were performed to determine metabolite profiles.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Using an orthogonal approach that combines untargeted metabolomics and biological data from a screening assay, we characterized the phytochemical profiles of the different extracts and prioritized samples for targeted isolation. The dichloromethane extract of\n                      <jats:italic>Sideritis cypria<\/jats:italic>\n                      exhibited a notable protective effect. Untargeted analysis revealed coumarins as key compounds, with varying amounts across\n                      <jats:italic>Sideritis<\/jats:italic>\n                      species. Accordingly, fractionation of extract resulted in the isolation of two coumarin derivatives. Structure elucidation was performed using one\u2010 and two\u2010dimensional nuclear magnetic resonance experiments. The coumarin, more abundant in\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>S. cypria<\/jats:italic>\n                      <\/jats:styled-content>\n                      , demonstrated a slight protective effect in the SARS\u2010CoV\u20102 pretreatment assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study highlights the antiviral effects of\n                      <jats:italic>Sideritis<\/jats:italic>\n                      taxa, although further investigations are necessary to clarify the full potential of the herb. Additionally, the methodology presented herein can serve as a valuable resource for future phytochemical investigations focused on coumarin content within\n                      <jats:italic>Sideritis<\/jats:italic>\n                      genus.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.3531","type":"journal-article","created":{"date-parts":[[2025,4,5]],"date-time":"2025-04-05T17:28:14Z","timestamp":1743874094000},"page":"1570-1579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted Isolation of Coumarins From\n                    <i>Sideritis<\/i>\n                    Species Based on Antiviral Screening and Untargeted Metabolomics"],"prefix":"10.1002","volume":"36","author":[{"given":"Ekaterina\u2010Michaela","family":"Tomou","sequence":"first","affiliation":[{"name":"Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences National and Kapodistrian University of Athens  Athens Greece"},{"name":"Organic\u2010Analytical Chemistry Weihenstephan\u2010Triesdorf University of Applied Sciences  Straubing Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Engler","sequence":"additional","affiliation":[{"name":"Spiez Laboratory Federal Office for Civil Protection  Spiez Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonios","family":"Chrysargyris","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, Biotechnology and Food Science Cyprus University of Technology  Limassol Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolaos","family":"Tzortzakis","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, Biotechnology and Food Science Cyprus University of Technology  Limassol Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2175-1407","authenticated-orcid":false,"given":"Helen","family":"Skaltsa","sequence":"additional","affiliation":[{"name":"Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8895-9416","authenticated-orcid":false,"given":"Corinna","family":"Urmann","sequence":"additional","affiliation":[{"name":"Organic\u2010Analytical Chemistry Weihenstephan\u2010Triesdorf University of Applied Sciences  Straubing Germany"},{"name":"TUM Campus Straubing for Biotechnology and Sustainability Technical University of Munich  Straubing Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102008\u20103"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00722-z"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.9b01285"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6575"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3259"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","unstructured":"N.Vahekeni J.Stehlin C.Urmann et\u00a0al. Historical Texts as a Potential Resource for Plant\u2010Derived Natural Products Against SARS\u2010CoV\u20102 \u2013 The Example of the Receptarium of Burkhard III von Hallwyl From 16th Century Switzerland. Published online June 13 (2024):2024.06.13.598798 https:\/\/doi.org\/10.1101\/2024.06.13.598798.","DOI":"10.1101\/2024.06.13.598798"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.117535"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metop.2021.100141"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9051583"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3088"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.03.014"},{"key":"e_1_2_6_13_1","unstructured":"European Union Herbal Monograph on Sideritis scardica Griseb.; Sideritis clandestina (Bory & Chaub.) Hayek; Sideritis raeseri Boiss. & Heldr.; Sideritis syriaca L. herba. Published online EMA\/HMPC\/39453 (2015). chrome\u2010extension:\/\/efaidnbmnnnibpcajpcglclefindmkaj\/https:\/\/www.ema.europa.eu\/en\/documents\/herbal\u2010monograph\/final\u2010european\u2010union\u2010herbal\u2010monograph\u2010sideritis\u2010scardica\u2010griseb\u2010sideritis\u2010clandestina\u2010bory\u2010chaub\u2010hayek\u2010sideritis\u2010raeseri\u2010boiss\u2010heldr\u2010sideritis\u2010syriaca\u2010l\u2010herba_en.pdf."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.01.022"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/13880209.2023.2280253"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25163763"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40199\u2010019\u201000261\u20108"},{"volume-title":"Planta Medica","year":"2019","author":"Pihan L. M.","key":"e_1_2_6_18_1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102006\u2010949890"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.25135\/rnp.163.19.08.1373"},{"key":"e_1_2_6_21_1","unstructured":"E. M.Tomou H.Skaltsa andC.Urmann.Unveiling Terpenoid Diversity in Sideritis spp. through Molecular Networks. In:S3\u2010P51 International Congress on Natural Products Research. (2024)."},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1055\/a\u20102072\u20102351"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2012.01.018"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1572-5995(06)80033-5"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010020\u20100700\u20103"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100033\u20101350809"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8110521"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202000966"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2014.07.005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25102382"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(81)85289\u20102"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031\u20109422(98)00731\u20106"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2019.112213"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(91)84149\u2010M"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.2874"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26113151"},{"issue":"2","key":"e_1_2_6_37_1","first-page":"170","article-title":"Asignaci\u00f3n De Espectros RMN 1 3 C De Cumarinas Mediante Correlaciones Heteronucleares Prot\u00f3n\u2010Carbono","volume":"82","author":"San Feliciano A.","year":"1986","journal-title":"An Qu\u00edm Ser C Qu\u00edm Org\u00e1nica Bioqu\u00edmica."},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/963248"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6492346"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21134618"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e03217"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2024.740703"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2021.e07777"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0194880"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.3531","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T17:04:42Z","timestamp":1761066282000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.3531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,4]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/pca.3531"],"URL":"https:\/\/doi.org\/10.1002\/pca.3531","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"type":"print","value":"0958-0344"},{"type":"electronic","value":"1099-1565"}],"subject":[],"published":{"date-parts":[[2025,4,4]]},"assertion":[{"value":"2024-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}