{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:03:12Z","timestamp":1774314192311,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2025,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n \n The present investigation of\n \n Nymphoides hydrophylla<\/jats:italic>\n <\/jats:styled-content>\n is a Taiwan indigenous edible aquatic plant, belongs to the Menyanthaceae family, and has been used as a traditional food with potential medicinal values against fever, insect bites, ulcer, and skin diseases. Interestingly, we found that the plant extract showed a significant anti\u2010inflammatory effect.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n The aim is isolation, structure elucidation, and anti\u2010inflammatory evaluation of secondary metabolites from\n \n N. hydrophylla<\/jats:italic>\n <\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Material and Methods<\/jats:title>\n This study involves the bioactive isolation and structural studies of the pure compounds determined by NMR, UV, IR spectroscopies, and HRMS. The anti\u2010inflammatory activity of the pure compounds was evaluated in\u00a0vitro. In addition, isolates showed remarkable anti\u2010inflammatory activities to regulate superoxide anion generation and elastase release in fMLF\/CB\u2010induced human neutrophils.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n This study conducted 10 oleanane\u2010type saponins with oligoglycoside linkage, including two new (\n 1<\/jats:bold>\n and\n 2<\/jats:bold>\n ) identified from the butanol extract of\n \n N. hydrophylla<\/jats:italic>\n <\/jats:styled-content>\n . As a result, Compounds\n 1<\/jats:bold>\n and\n 2<\/jats:bold>\n , representing new triterpene saponins, have not been previously reported. In\u00a0vitro anti\u2010inflammatory tests revealed that Compounds\n 1<\/jats:bold>\n ,\n 5<\/jats:bold>\n \u2013\n 7<\/jats:bold>\n ,\n 9<\/jats:bold>\n , and\n 10<\/jats:bold>\n showed significant anti\u2010inflammatory effects by inhibiting superoxide anion generation with IC\n 50<\/jats:sub>\n values of 1.62, 0.92, 0.28, 2.69, 0.29, and 0.15\u2009\u03bcM, respectively, whereas compounds\n 1<\/jats:bold>\n ,\n 5<\/jats:bold>\n ,\n 6<\/jats:bold>\n ,\n 9<\/jats:bold>\n , and\n 10<\/jats:bold>\n showed potential inhibition on elastase release in human neutrophils, with IC\n 50<\/jats:sub>\n values of 0.19, 1.12, 0.70, 0.68, and 0.51\u2009\u03bcM, respectively.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Our findings suggested that the components of\n \n N. hydrophylla<\/jats:italic>\n <\/jats:styled-content>\n have the potential to develop anti\u2010inflammatory agents.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.3539","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T04:29:31Z","timestamp":1747801771000},"page":"1710-1718","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti\u2010Inflammatory Saponins From an Edible Aquatic Plant\n \n Nymphoides hydrophylla<\/i>\n <\/scp>"],"prefix":"10.1002","volume":"36","author":[{"given":"Ching\u2010Yeu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Physical Therapy Tzu\u2010Hui Institute of Technology Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu\u2010Peng","family":"Cheng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Natural Products and Graduate Institute of Biomedical Sciences, College of Medicine Chang Gung University Taoyuan 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