{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T19:29:31Z","timestamp":1767900571999,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,8,17]],"date-time":"2001-08-17T00:00:00Z","timestamp":998006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new, fast and reliable procedure for the quantification of the major compounds of <jats:italic>Hypericum perforatum<\/jats:italic> L. has been developed. Four naphthodianthrones (protopseudohypericin, pseudohypericin, protohypericin, hypericin) and two phloroglucinols (hyperforin, adhyperforin) were assayed by HPLC using a short (17 min) linear gradient, with hypericin and hyperforin as external standards. Extraction of dried plant material with methanol in the dark at room temperature for 2\u2005h led to a complete recovery of phloroglucinols but only a partial recovery of the naphthodianthrone derivatives. Treatment of plant material with water:ethanol (40:60, v\/v) in a water bath shaker at 80\u00b0C led to the total extraction of hypericins, but a 10% loss of total hyperforins was also observed. The two extraction methods, applied successively to the same sample, allowed the complete extraction of all compounds of interest. A 5\u2005min exposure of the crude extract of <jats:italic>H. perforatum<\/jats:italic> to sunlight (1 E\/m<jats:sup>2<\/jats:sup>) induced a 96% loss of hyperforins, whereas the dry plant material lost only 20% of hyperforins after 2\u2005h exposure to sunlight (24 E\/m<jats:sup>2<\/jats:sup>). Copyright \u00a9 2001 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.601","type":"journal-article","created":{"date-parts":[[2005,1,25]],"date-time":"2005-01-25T04:35:55Z","timestamp":1106627755000},"page":"355-362","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Improved procedure for the quality control of<i> Hypericum perforatum<\/i> L."],"prefix":"10.1002","volume":"12","author":[{"given":"Anne","family":"Poutaraud","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelise","family":"Lobstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Girardin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Weniger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,8,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00697-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-957437"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-979340"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)00506-8"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00407-7"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-1999-14050"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-979339"},{"key":"e_1_2_1_9_1","unstructured":"ErdelmeierCAJ KlessingK RenziS HauerH.1999.New hyperforin analogues fromHypericum perforatumand a stable dicyclohexylammonium salt of hyperforin. 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