{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:29:39Z","timestamp":1772998179075,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2021423066"],"award-info":[{"award-number":["H2021423066"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104358"],"award-info":[{"award-number":["82104358"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074127"],"award-info":[{"award-number":["82074127"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015800","name":"Jilin Province Development and Reform Commission","doi-asserted-by":"publisher","award":["2023C027\u20102"],"award-info":[{"award-number":["2023C027\u20102"]}],"id":[{"id":"10.13039\/100015800","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Dioscoreae Rhizoma<\/jats:italic>\n                      (DR) is a plant recognized for its dual medicinal and edible applications, exhibiting notable therapeutic efficacy, particularly in the treatment of postmenopausal osteoporosis (PMOP) associated with estrogen deficiency.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study sought to systematically identify bioactive compounds present in DR that interact with estrogen receptor \u03b2 (ESR2) and employ affinity ultrafiltration in conjunction with UPLC\u2010QE\u2010Orbitrap\u2010MS to find possible therapy options for PMOP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, a C18 column was employed to fractionate the DR extract into distinct fractions, and the optimal active site in DR was identified based on its osteoprotegerin (OPG) content in MC3T3\u2010E1 cells. To identify the DR components exhibiting high binding affinity for ESR2, affinity ultrafiltration coupled with UPLC\u2010QE\u2010Orbitrap\u2010MS was utilized. These findings were further corroborated through molecular docking and molecular dynamics simulations. To further validate the osteogenic effects of the identified compounds, CCK\u20108 proliferation assays, along with OPG and alkaline phosphatase (ALP) activity assays, were employed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The 30% DR fraction demonstrated significant anti\u2010PMOP activity. Acacetin, Adenosine, and Procyanidin B2 are recognized as the principal active constituents responsible for the anti\u2010PMOP effects of DR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study introduces a comprehensive approach combining affinity ultrafiltration with UPLC\u2010QE\u2010Orbitrap\u2010MS and molecular docking to efficiently identify ESR2\u2010targeted therapeutic compounds for PMOP in DR. The findings offer both theoretical and empirical foundations for the advancement of novel therapeutic strategies for PMOP.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.70034","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T11:24:50Z","timestamp":1760181890000},"page":"216-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening of Anti\u2010Postmenopausal Osteoporosis Active Components With ESR2 Targeting Affinity in\n                    <i>Dioscoreae Rhizoma<\/i>\n                    Based on Affinity Ultrafiltration\u2010UPLC\u2010QE\u2010Orbitrap\u2010MS"],"prefix":"10.1002","volume":"37","author":[{"given":"Wanjie","family":"Liu","sequence":"first","affiliation":[{"name":"Jilin Ginseng Academy Changchun University of Chinese Medicine  Changchun 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