{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T23:29:42Z","timestamp":1783639782661,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204760"],"award-info":[{"award-number":["82204760"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Evodia<\/jats:italic>\n                      fructus (EF) is a traditional Chinese medicine with a long history of medicinal use. However, its slight hepatotoxicity hindered the development of its drug safety profile. Consequently, it was essential to elucidate the composition of its chemical components, as well as its absorption and metabolism within the body.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We aimed to conduct a comprehensive and systematic analysis of the chemical components of EF, as well as the constituents present in\u00a0vivo following administration to rats. Additionally, to refine the research on the identification of EF compounds and speculate on their potential medicinal ingredients, the mass spectrometry cleavage pathways and in\u00a0vivo metabolic pathways of various compound types were summarized.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ultra\u2010high performance liquid chromatography\u2010quadrupole time\u2010of\u2010flight mass spectrometry (UPLC\u2010Q\u2010TOF\u2010MS) was employed to qualitatively analyze the components and metabolites of EF in\u00a0vitro and in\u00a0vivo. The compounds were identified using MassLynx 4.1 software, which facilitated the analysis of retention time, precise molecular mass, and both primary and secondary spectrometry data.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 93 constituents were detected from the ethanol extract of EF. Moreover, 73 prototype constituents and 66 metabolites were observed and inferred from the plasma, urine, and feces of rats following administration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The chemical compounds of EF can be primarily categorized into alkaloids, flavonoids, terpenoids, and phenylpropanoids. The predominant compounds present in\u00a0vivo were the prototype components, and the metabolites were mostly alkaloids and terpenoids. The metabolic pathways involved included hydroxylation, hydrogenation, methylation, and glucuronide conjugation, etc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.70036","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T05:56:16Z","timestamp":1763013376000},"page":"241-272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comprehensive Analysis of Chemical Composition and In\u00a0Vivo Metabolic Pathways of\n                    <i>Evodia<\/i>\n                    Fructus Using UPLC\u2010Q\u2010TOF\u2010MS"],"prefix":"10.1002","volume":"37","author":[{"given":"Mengru","family":"Wu","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenna","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehan","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjiao","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangzhi","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4760-101X","authenticated-orcid":false,"given":"Yaqi","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115495"},{"key":"e_1_2_7_3_1","unstructured":"Zang BS.Analysis of Ingredients FromEvodia rutaecarpaIn\u00a0Vivo Based on Hepatotoxicity. (2016)."},{"key":"e_1_2_7_4_1","volume-title":"Pharmacopoeia of the People's Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/HM9.0000000000000108"},{"issue":"11","key":"e_1_2_7_6_1","first-page":"2953","article-title":"Characterization and Identification of Chemical Components From Euodiae Fructus Based on UHPLC\u2010Q\u2010TOF\u2010MS","volume":"49","author":"Wang Y.","year":"2024","journal-title":"China Journal of Chinese Materia Medica."},{"issue":"06","key":"e_1_2_7_7_1","first-page":"113","article-title":"Analysis of Major Bioactive Components and Their Metabolites in Rats' Plasma, Urine and Feces After Oral Administration of the Aqueous Extract of Euodiae Fructus by UHPLC\u2010QTOF\u2010MS","volume":"27","author":"Zhao X. 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