{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:51:17Z","timestamp":1790182277243,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by persistent inflammation, synovial hyperplasia, and osteochondral damage.\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Murraya paniculata<\/jats:italic>\n                      <\/jats:styled-content>\n                      (L.) Jack (MP), a botanical source of Murrayae Folium et Cacumen (MFC), has not previously been investigated for its potential arthritis\u2010protective effects and underlying mechanisms, prompting our study of MP in this context.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study is aimed at evaluating the ameliorating effects of MP and elucidating its underlying mechanisms in RA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The chemical composition of MP extract was analyzed using ultra\u2010performance liquid chromatography (UPLC). The anti\u2010arthritic effects of MP extract were assessed in collagen\u2010induced arthritis (CIA) rats and interleukin (IL)\u20101\u03b2\u2010stimulated SW982 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>UPLC quantification identified four major constituents: luteolin tetramethylether (0.547 \u00b1 0.012%), 5,7,3\u2032,4\u2032,5\u2032\u2010pentamethoxyflavone (1.558 \u00b1 0.030%), 3\u2032,4\u2032,5,5\u2032,6,7\u2010hexamethoxyflavone (0.273 \u00b1 0.006%), and 5\u2010demethylnobiletin (0.848 \u00b1 0.023%). In\u00a0vivo, MP alleviated arthritis in CIA rats by reducing clinical symptoms (including arthritis index, hind paw volume\/swelling, and histopathological joint damage), suppressing inflammatory responses, and attenuating oxidative stress. In\u00a0vitro, MP mitigated pathological changes in IL\u20101\u03b2\u2010stimulated SW982 cells by inhibiting cell proliferation and migration, dampening inflammation, and reducing oxidative stress. Furthermore, network pharmacology and transcriptomic analyses guided the investigation of MP's regulatory effects on key RA\u2010related targets. Specifically, MP downregulated IL\u20101\u03b2\u2010induced overexpression of iNOS, COX\u20102, and MMP2\/9 in SW982 cells and inhibited AP\u20101\/NF\u2010\u03baB activation. These effects were confirmed in\u00a0vivo, where MP suppressed MMP2\/9 expression and blocked AP\u20101\/NF\u2010\u03baB activation in CIA rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Integrating phytochemical profiling, in\u00a0vivo and in\u00a0vitro models, network pharmacology\u2010transcriptomics, and target validation, this study demonstrates that MP exerts anti\u2010arthritic effects via modulating proliferation\u2013inflammation\u2013oxidation crosstalk.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.70048","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T14:29:56Z","timestamp":1768487396000},"page":"414-431","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ameliorating Effects of\n                    <scp>\n                      <i>Murraya paniculata<\/i>\n                    <\/scp>\n                    (L.) Jack Extract on Arthritis via Suppressing Multi\u2010Target\u2010Mediated Synovial Hyperplasia, Inflammation, and Oxidative Stress"],"prefix":"10.1002","volume":"37","author":[{"given":"Guoping","family":"Wu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences University of Macau  Taipa Macao SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences University of Macau  Taipa Macao SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junming","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences University of Macau  Taipa Macao SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiushuo","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences University of Macau  Taipa Macao SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences University of Macau  Taipa Macao SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Xie","sequence":"additional","affiliation":[{"name":"China Resources Sanjiu Medical &amp; Pharmaceutical Co., Ltd.  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9466-6721","authenticated-orcid":false,"given":"Hua","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mechanism and Quality of Chinese Medicine, Institute of Chinese Medical Sciences University of Macau  Taipa Macao SAR China"},{"name":"Department of Pharmaceutical Sciences, Faculty of Health Sciences University of Macau  Taipa Macao SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,15]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.547913"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.70037"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787\u2010024\u201001445\u20108"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3479"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2019.102400"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2024.04.004"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010020\u201000372\u20104"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2024.176551"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075\u2010019\u20101935\u20106"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S398690"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2017.04.012"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3221"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3508"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1337161"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114567"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.464736"},{"key":"e_1_2_11_18_1","first-page":"1947","article-title":"Coumarins and Flavonoid From Murraya paniculata (L.) 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