{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:46:47Z","timestamp":1785815207863,"version":"3.56.0"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>A traditional Chinese medicine Yi\u2010Qi\u2010Jian\u2010Pi\u2010Xiao\u2010Yu (YQJPXY) formula can potentially ameliorate chronic kidney disease (CKD), but its effect on renal fibrosis (RF), a prevalent pathological feature of CKD, remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To mimic renal injury and fibrosis, a mouse unilateral ureteral obstruction (UUO) model was employed. Using hematoxylin\u2013eosin and Masson's trichrome stains, enzyme\u2010linked immunosorbent assay, and immunofluorescence, the effects of YQJPXY on renal injury in UUO mice were evaluated. A series of bioinformatics analyses were then conducted to explore the key genes and immune cells involved in the therapeutic mechanism of YQJPXY. Finally, in\u00a0vivo and in\u00a0vitro studies provided multifaceted evaluations of YQJPXY against RF from the perspectives of macrophage dynamics and roles of HIF1A.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      YQJPXY treatment significantly reduced levels of TGF\u2010\u03b21 and IL\u20106, attenuated tubular damage, decreased extracellular matrix protein (FN and Col\u2010I) expressions, and modulated fibrosis and epithelial\u2010mesenchymal transition markers in UUO mice in a dose\u2010dependent manner. Bioinformatics analysis identified HIF1A as the key target of YQJPXY against RF. In\u00a0vivo, HIF1A deficiency lessened RF and recruitment of CD45\n                      <jats:sup>+<\/jats:sup>\n                      \u2009CD11b\n                      <jats:sup>+<\/jats:sup>\n                      \u2009and CD45\n                      <jats:sup>+<\/jats:sup>\n                      \u2009F4\/80\n                      <jats:sup>+<\/jats:sup>\n                      \u2009macrophages in fibrotic kidneys. During treatment, YQJPXY inhibited HIF1A expression and reduced M1 macrophage polarization. In\u00a0vitro experiments confirmed that YQJPXY suppresses hypoxia\u2010induced M1 macrophage polarization and HK\u20102 cell transdifferentiation via downregulating HIF1A.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>YQJPXY alleviates RF by attenuating HIF1A\u2010mediated M1 macrophage polarization, providing a potential therapeutic approach for CKD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.70077","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:49:46Z","timestamp":1783687786000},"page":"813-825","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antifibrotic Effects of Yi\u2010Qi\u2010Jian\u2010Pi\u2010Xiao\u2010Yu Formula in Kidney via HIF1A\u2010Driven M1 Macrophage Polarization"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6874-4405","authenticated-orcid":false,"given":"Keda","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Nephrology Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Nephrology The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenze","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou 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