{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T10:00:41Z","timestamp":1792404041835,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to develop and validate a UHPLC\u2010MS\/MS method for the simultaneous determination of 14 constituents related to Renshen Guben Oral Liquid (RSGB) in human plasma and to explore concentration\u2013absolute neutrophil count (ANC) associations in cancer patients with chemoradiotherapy\u2010induced neutropenia (CIN).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Candidate constituents were selected by integrating chemical representativeness, disease relevance, reference standard availability, and analytical feasibility, with support from network pharmacology. Plasma samples were collected from 25 patients before administration and at 1 and 2\u2009h after RSGB administration. Samples were processed by liquid\u2013liquid extraction using methyl tert\u2010butyl ether or n\u2010butanol and analyzed by UHPLC\u2010MS\/MS. Spearman correlation analysis was used to assess exploratory associations between post\u2010dose concentrations of major quantifiable circulating constituents and ANC change from baseline to Day 15.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The assay fulfilled predefined bioanalytical acceptance criteria and enabled simultaneous quantification of 14 RSGB\u2010related constituents in human plasma. Ginsenoside Rb1 (G\u2010Rb1), ginsenoside compound K (G\u2010CK), and paeonol (PAE) were the major quantifiable circulating constituents, whereas protopanaxadiol (PPD) and protopanaxatriol (PPT) were only sporadically quantifiable. Exploratory analyses showed positive associations between concentrations of G\u2010Rb1, G\u2010CK, and PAE and \u0394ANC. Molecular docking provided complementary computational support for potential target interactions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>G\u2010Rb1, G\u2010CK, and PAE were the major quantifiable circulating RSGB\u2010related constituents within the early sampling window, and their concentrations showed exploratory associations with ANC change. These findings provide complementary in\u00a0vivo evidence for evaluating RSGB\u2010related constituents.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.70089","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T08:58:01Z","timestamp":1789549081000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["UHPLC\u2010MS\/MS\u2010Based Quantification of 14 Renshen Guben Oral Liquid\u2013Related Constituents in Cancer Patients With Neutropenia"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4551-5560","authenticated-orcid":false,"given":"Mengyuan","family":"Wu","sequence":"first","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaojie","family":"Ji","sequence":"additional","affiliation":[{"name":"Qingdao Central Hospital, University of Health and Rehabilitation Sciences  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjiang","family":"Dong","sequence":"additional","affiliation":[{"name":"Wulian County Medical and Health Industry Development Service Center  Rizhao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8177-4887","authenticated-orcid":false,"given":"Sitong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4816-6010","authenticated-orcid":false,"given":"Shuo","family":"Diao","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xintong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boya","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da","family":"Rong","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enli","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine Lunan Pharmaceutical Group Co., Ltd  Linyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Niu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine Lunan Pharmaceutical Group Co., Ltd  Linyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4179-0245","authenticated-orcid":false,"given":"He","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine Lunan Pharmaceutical Group Co., Ltd  Linyi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9504-891X","authenticated-orcid":false,"given":"Mingming","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Lv","sequence":"additional","affiliation":[{"name":"School of Medicine and Pharmacy Ocean University of China  Qingdao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherwin K. 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