{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:55:29Z","timestamp":1790999729964,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3501700"],"award-info":[{"award-number":["2022YFC3501700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3501704"],"award-info":[{"award-number":["2022YFC3501704"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3501701"],"award-info":[{"award-number":["2022YFC3501701"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Aim<\/jats:title>\n                    <jats:p>\n                      Practical implementation of integrated chemical\u2013biological quality control in Traditional Chinese Medicine (TCM) remains challenging. This study used\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Salvia miltiorrhiza<\/jats:italic>\n                      <\/jats:styled-content>\n                      as a model to develop an exploratory integrated quality\u2010evaluation strategy that combines chemical profiling with a standardized in\u00a0vitro relative\u2010potency assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Chemical characterization of 58 batches included UPLC fingerprinting and quantification of 10 constituents. Relative biological potency was evaluated using a parallel\u2010line assay in an H9c2 cardiomyoblast oxygen\u2010glucose deprivation\/reoxygenation (OGD\/R) model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Across the 58 commercial batches, fingerprint similarity ranged from 0.469 to 0.997 and the total content of the 10 quantified compounds ranged from 1.36% to 8.27%. Total tanshinones showed greater interbatch variation (CV\u2009=\u200955.2%) than total phenolic acids (CV\u2009=\u200946.2%). Four batches did not satisfy the statistical validity criteria of the parallel\u2010line model and were therefore nonreportable; two of these batches showed no conspicuous abnormality in the routine quantitative indicators. Among the 54 reportable batches, relative potency ranged from 57.6 to 276.5\u2009U\/mg and correlated moderately with total quantified content (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.719,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Partial least squares regression identified salvianolic acid B, rosmarinic acid, cryptotanshinone, and tanshinone IIA as chemical variables statistically associated with the in\u00a0vitro response. The median\u2010based four\u2010quadrant analysis flagged chemical\u2013biological discordance in 10 of 54 batches (18.5%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The integration of chemical profiling with a standardized cell\u2010based relative potency assay enabled multidimensional assessment of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>S. miltiorrhiza<\/jats:italic>\n                      <\/jats:styled-content>\n                      quality by simultaneously characterizing batch composition, biological response, and their concordance. This approach provides a practical basis for identifying quality variations that may not be captured by chemical analysis alone and supports the development of more comprehensive quality\u2010evaluation strategies for botanical drugs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pca.70091","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:26:13Z","timestamp":1790997973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Construction and Application of an Integrated \u201cChemical\u2013Biological\u201d Dual\u2010Core Quality Evaluation System for\n                    <scp>\n                      <i>Salvia miltiorrhiza<\/i>\n                    <\/scp>"],"prefix":"10.1002","author":[{"given":"Xiaoyue","family":"Min","sequence":"first","affiliation":[{"name":"Wuya College of Innovation Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianyue","family":"Liang","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huali","family":"Long","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjun","family":"Hou","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9411-6358","authenticated-orcid":false,"given":"De\u2010an","family":"Guo","sequence":"additional","affiliation":[{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"Shanghai Academy of International Standardization for Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9549-6448","authenticated-orcid":false,"given":"Wanying","family":"Wu","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation Shenyang Pharmaceutical University  Shenyang China"},{"name":"Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica, Chinese Academy of Sciences  Shanghai China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"U.S. Food and Drug Administration \u201cBotanical Drug Development: Guidance for Industry \u201d Silver Spring MD US Department of Health and Human Services Food and Drug Administration 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