{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:53:43Z","timestamp":1791194023190,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"abstract":"ABSTRACT<\/jats:title>\n \n Objective<\/jats:title>\n This study aims to systematically evaluate the in\u00a0vitro stability and pharmacokinetic behavior of representative polyphenols in Qingzhuan dark tea (QDT) and to explore the relationship between physicochemical stability and systemic exposure.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Twelve representative polyphenols, including eight catechins and four theaflavins, were evaluated using a three\u2010factor, three\u2010level L\n 9<\/jats:sub>\n orthogonal design to investigate the effects of pH, temperature, and time on stability. Based on stability and plasma detectability, seven catechins were further investigated in male Sprague\u2013Dawley rats following oral administration of QDT extract at doses of 1, 2, and 3\u2009g\/kg. A validated liquid chromatography\u2013mass spectrometry (LC\u2010MS) method was used for quantitative analysis and pharmacokinetic evaluation.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The optimal preservation condition for the QDT extract and mixed standards was pH\u20095, 4\u00b0C, and 30\u2009min. The analytical method showed satisfactory linearity (\n r<\/jats:italic>\n \u2009>\u20090.9908), precision (<\u20099.82%), accuracy (90.2%\u2013109.8%), recovery (63.9%\u201390.8%), and matrix effects (87.64%\u2013108.76%). Seven catechins were detected in rat plasma, whereas theaflavins were not detected under the present experimental conditions. C\n max<\/jats:sub>\n and AUC generally increased with increasing dose, indicating dose\u2010dependent systemic exposure.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The integrated stability and pharmacokinetic evaluation establishes a link between physicochemical stability and biological disposition of QDT polyphenols, providing a scientific basis for QDT storage optimization, quality control, formulation development, and functional application.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/pca.70092","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:12:28Z","timestamp":1791191548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Stability and Pharmacokinetic Evaluation of 12 Compounds in Qingzhuan Dark Tea in Rat Models"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4252-0409","authenticated-orcid":false,"given":"Xiaolei","family":"Miao","sequence":"first","affiliation":[{"name":"School of Pharmacy Xianning Medical College, Hubei University of Science and Technology Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"Quan","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xianning Medical College, Hubei University of Science and Technology Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Ye","sequence":"additional","affiliation":[{"name":"Nanchong College of Science and Technology School of Medicine Nanchong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy Xianning Medical College, Hubei University of Science and Technology Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiye","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Third People's Hospital of Jiujiang Jiujiang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1080\/07315724.2020.1827082","article-title":"Potential Bioactive Components and Health Promotional Benefits of Tea (Camellia sinensis)","volume":"41","author":"Samanta S.","year":"2022","journal-title":"Journal of the American Nutrition Association"},{"issue":"9","key":"e_1_2_9_3_1","doi-asserted-by":"crossref","DOI":"10.3390\/antiox11091655","article-title":"Antioxidant Capacities and Polyphenol Contents of Kombucha Beverages Based on Vine Tea and Sweet Tea","volume":"11","author":"Saimaiti 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