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Total RNA was extracted <jats:italic>via<\/jats:italic> several purification steps designed to minimize binding of phenolics to nucleic acids and was then subjected to caesium chloride ultra\u2010centrifugation. This procedure produced consistently high\u2010quality, intact RNA from both needles and roots with spectrophotometric ratios of approximately 2.0 for both 260\/280\u2005nm and 260\/230\u2005nm. Total RNA was obtained from the roots of cold\u2010hardened white spruce seedlings for cDNA library construction. More than 2 million recombinant phage particles were generated from 5\u2005\u00b5g of a poly(A)+RNA fraction, and ca. 1.3 million cDNA particles were amplified for storage. Approximately 500,000 primary recombinant clones were screened with an heterologous PinmIII cDNA sequence yielding a unique clone, picg1, that was very similar to members of the PR10 gene family. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.701","type":"journal-article","created":{"date-parts":[[2003,7,14]],"date-time":"2003-07-14T06:09:15Z","timestamp":1058162955000},"page":"209-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Isolation of high\u2010quality RNA from white spruce tissue using a three\u2010stage purification method and subsequent cloning of a transcript from the PR\u201010 gene family"],"prefix":"10.1002","volume":"14","author":[{"given":"Nathalie","family":"Mattheus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abul K. 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