{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:04:45Z","timestamp":1791115485927,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T00:00:00Z","timestamp":1119398400000},"content-version":"vor","delay-in-days":52,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The dichloromethane extract of the aerial parts of <jats:italic>Blumea gariepina<\/jats:italic> (Asteraceae) was shown to be active against the phytopathogenic fungus <jats:italic>Cladosporium cucumerinum<\/jats:italic> and to inhibit acetylcholinesterase. In order rapidly to identify the active principles, the crude extract was analysed by on\u2010flow HPLC\u2010<jats:sup>1<\/jats:sup>H\u2010NMR. HPLC\u2010micro\u2010fractionation was performed and all peaks collected were submitted to assays against <jats:italic>C. cucumerinum<\/jats:italic> and acetylcholinesterase. By this means, the biological activities could be efficiently associated with selected HPLC peaks. Complementary on\u2010line structural data for all peaks of interest in the crude extract were obtained from HPLC\u2010MS and from HPLC\u2010UV with post\u2010column addition of UV shift reagents. This chemical screening strategy with integrated bioassays permitted the on\u2010line identification of a number of constituents and gave useful information for an efficient isolation procedure. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.839","type":"journal-article","created":{"date-parts":[[2005,6,22]],"date-time":"2005-06-22T13:47:36Z","timestamp":1119448056000},"page":"166-174","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["On\u2010line identification of the bioactive compounds from <i>Blumea gariepina<\/i> by HPLC\u2010UV\u2010MS and HPLC\u2010UV\u2010NMR, combined with HPLC\u2010micro\u2010fractionation"],"prefix":"10.1002","volume":"16","author":[{"given":"E. 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