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The study was performed on three different species of <jats:italic>Trifolium<\/jats:italic> (Leguminosae), namely <jats:italic>Trifolium pratense<\/jats:italic> L., <jats:italic>T. pallescens<\/jats:italic> Schreb. and <jats:italic>T. alpinum<\/jats:italic> L, collected in Switzerland. The comparison between the dichloromethane extracts revealed that the main aglycones are present in the three species whereas the methanolic extracts show different glycosides and malonate derivatives. The compounds of interest were mainly flavonoids, isoflavonoids and clovamides. Their identities were confirmed from retention times, UV and MS analyses and UV shifts following post\u2010column derivatisation. 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