{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:19:32Z","timestamp":1790788772326,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T00:00:00Z","timestamp":1163376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Soluble waxes were extracted from the cuticle of ivy (<jats:italic>Hedera helix<\/jats:italic> L.) leaves with dichloromethane in a yield of ca. 13%. The cuticular waxes were directly analysed by GC\u2010MS, high\u2010temperature GC\u2010MS and ESI\u2010MS\/MS. The GC\u2010MS analysis showed mostly <jats:italic>n<\/jats:italic>\u2010alkanols (45.3%), monoacids (18.8%), triterpenes (9.7%), <jats:italic>n<\/jats:italic>\u2010aldehydes (8.7%) and <jats:italic>n<\/jats:italic>\u2010alkanes (7.7%). The high\u2010temperature GC\u2010MS and the ESI\u2010MS\/MS analyses showed the presence of ester waxes, namely alkyl alkanoates and alkyl coumarates. Alkyl alkanoates comprised esters of the hexadecanoic acid with <jats:italic>n<\/jats:italic>\u2010alkanols ranging from C16 to C34. Alkyl coumarates included esters of coumaric acid with <jats:italic>n<\/jats:italic>\u2010alkanols ranging from C16 to C32. The cuticular waxes were hydrolysed and the resulting organic and aqueous phases analysed by GC\u2010MS. The hydrolysate showed a major increase in the quantities of <jats:italic>n<\/jats:italic>\u2010alkanols, hexadecanoic acid and coumaric acid derived from the alkyl and acyl moieties from the ester waxes. A content of ester waxes of 38% was estimated based on the results from the GC\u2010MS analysis of the non\u2010hydrolysed and hydrolysed cuticular waxes. Alkyl alkanoates were analysed by ESI\u2010MS\/MS as [M + Li]<jats:sup>+<\/jats:sup> adduct ions and the alkyl coumarates as [M \u2212 H]<jats:sup>\u2212<\/jats:sup> deprotonated ions. The ESI\u2010MS\/MS analysis allowed the detection of a wider range of ester waxes than high\u2010temperature GC\u2010MS, and was shown to be a useful technique for the qualitative analysis of ester waxes from plant cuticles. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.952","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T19:39:24Z","timestamp":1163446764000},"page":"60-69","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Cuticular waxes from Ivy leaves (<i>Hedera helix<\/i> L.): analysis of high\u2010molecular\u2010weight esters"],"prefix":"10.1002","volume":"18","author":[{"given":"Sara","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Gra\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531236"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1677"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac025637a"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(02)00212-1"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004250050456"},{"key":"e_1_2_1_7_1","first-page":"1","volume-title":"Plant Epicuticular Waxes: Function, Production, and Genetics","author":"Jenks M","year":"1999"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(96)90007-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.1997.tb00684.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(02)00045-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004250050228"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1565(199805\/06)9:3<112::AID-PCA400>3.0.CO;2-A"},{"key":"e_1_2_1_13_1","first-page":"189","volume-title":"Plant Cuticles","author":"Riederer M","year":"1996"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/52.363.2023"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.2780420308"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00047a041"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.3358\/shokueishi.46.198"},{"key":"e_1_2_1_18_1","first-page":"235","volume-title":"Chemistry and Biochemistry of Natural Waxes","author":"Tulloch AP","year":"1976"},{"key":"e_1_2_1_19_1","first-page":"105","article-title":"Waxes, cutin and suberin","volume":"4","author":"Walton TJ","year":"1990","journal-title":"Meth Plant Biochem"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.952","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:29:47Z","timestamp":1761056987000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11,13]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/pca.952"],"URL":"https:\/\/doi.org\/10.1002\/pca.952","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11,13]]},"assertion":[{"value":"2006-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-11-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}