{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:12:59Z","timestamp":1791187979644,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,4,3]],"date-time":"2007-04-03T00:00:00Z","timestamp":1175558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytochemical Analysis"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Betula alleghaniensis<\/jats:italic> and <jats:italic>B. papyrifera<\/jats:italic> are widely distributed in the province of Qu\u00e9bec (Canada) and, since these trees are valuable exports for the local lumber industry, large amounts of their residual ligneous biomass are available for further exploitation. <jats:italic>Betula<\/jats:italic> species are well known for their significant concentrations of triterpenes, some of which were recently discovered to present promising bioactivity. The secondary transformation of birch biomass could therefore become important for many industries, particularly the pharmaceutical industry. In the present study, extracts from birch sawdust were obtained using an optimised ultrasound\u2010assisted extraction in which the careful choice of temperature permitted a selective extraction of the targeted triterpenes. Moreover, compared with the classical Soxhlet method, higher extraction yields were obtained in a shorter time. The lipophilic extracts obtained using dichloromethane as a solvent were analysed by GC\u2010MS and the major compounds identified as lupane\u2010type cyclic triterpenoids accompanied by the non\u2010cyclic triterpene squalene. Numerous aliphatic long\u2010chain fatty acids were also found in the extracts together with phytosterols. Betulonic acid and squalene, the major extract constituents for both <jats:italic>B. alleghaniensis<\/jats:italic> and<jats:italic> B. papyrifera<\/jats:italic>, are both bioactive molecules. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/pca.981","type":"journal-article","created":{"date-parts":[[2007,4,3]],"date-time":"2007-04-03T11:29:16Z","timestamp":1175599756000},"page":"291-299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Selective ultrasound\u2010assisted extractions of lipophilic constituents from <i>Betula alleghaniensis<\/i> and <i>B. papyrifera<\/i> wood at low temperatures"],"prefix":"10.1002","volume":"18","author":[{"given":"J.\u2010M.","family":"Lavoie","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Stevanovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,4,3]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"Identification of Essential oil Components by Gas Chromatography\/Quadrupole Mass Spectroscopy","author":"Adams RP","year":"2001"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.3183\/NPPRJ-2003-18-02-p129-133"},{"key":"e_1_2_1_4_1","first-page":"209","article-title":"Triterpenoid constituents in the outer bark of Betula alleghaniensis (yellow bich)","volume":"11","author":"Cole BJW","year":"1991","journal-title":"Wood Sci"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(04)00124-X"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-6031(02)00358-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1081\/WCT-120013354"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(86)90141-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/HF.2006.086"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.07.025"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf990146l"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(98)00295-9"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf050301y"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/HF.2006.030"},{"key":"e_1_2_1_15_1","unstructured":"MarquezL AgueroJ HernandezI GarridoG MartinezI DieguezR PrietoS RivasY Molina\u2010TorresJ CuriniM DelgadoR.2005.Anti\u2010inflammatory evaluation and phytochemical characterisation of some plants of theZanthoxylumgenus. Acta Farmaceutica Bonaerense24:325\u2013330."},{"key":"e_1_2_1_16_1","first-page":"911","article-title":"Emulsifiers of plant origin. I. Extraction of betulin from birch bark (Betula alba) with trichloroethylene","volume":"20","author":"Pasich J","year":"1964","journal-title":"Farm Polska"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.07.113"},{"key":"e_1_2_1_18_1","first-page":"17","article-title":"Complex study of Betula pendula growing in Russia","volume":"3","author":"Pervyshina GG","year":"2002","journal-title":"Khimiya Rastitel'nogo Syr'ya"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-951725"},{"key":"e_1_2_1_20_1","first-page":"1057","volume-title":"Natural Products of Woody Plants I and II.","author":"Rowe JW","year":"1991"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.ultsonch.2003.09.005","article-title":"Comparison of classical and ultrasound\u2010assisted extractions of steroids and triterpenoids from 3 Chresta spp","volume":"11","author":"Schinor EC","year":"2004","journal-title":"Ultrasonics Sonochem"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/np049938m"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200590045"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/np020379y"}],"container-title":["Phytochemical Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpca.981","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pca.981","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:25:50Z","timestamp":1761056750000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pca.981"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,3]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1002\/pca.981"],"URL":"https:\/\/doi.org\/10.1002\/pca.981","archive":["Portico"],"relation":{},"ISSN":["0958-0344","1099-1565"],"issn-type":[{"value":"0958-0344","type":"print"},{"value":"1099-1565","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,3]]},"assertion":[{"value":"2006-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}