{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:15:34Z","timestamp":1761707734599},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T00:00:00Z","timestamp":1101254400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Fetal cells cross the feto\u2010maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto\u2010maternal barrier changes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of fetal hemoglobin gamma chain messenger RNA (HbF\u2010\u03b3 mRNA) was measured by quantitative reverse transcriptase\u2010polymerase chain reaction (RT\u2010PCR) in maternal peripheral blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HbF\u2010\u03b3 mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF\u2010\u03b3 mRNA expression was significantly higher than in normal pregnancies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of HbF\u2010\u03b3 mRNA relative to that of \u03b2\u2010actin mRNA is thought to reflect the real\u2010time leakage of fetal cells into maternal blood. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.1039","type":"journal-article","created":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T16:52:01Z","timestamp":1101315121000},"page":"881-886","source":"Crossref","is-referenced-by-count":2,"title":["Quantitative analysis of cellular fetal hemoglobin gamma chain messenger RNA (HbF\u2010\u03b3 mRNA) in maternal peripheral blood"],"prefix":"10.1002","volume":"24","author":[{"given":"Tomohiro","family":"Okuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Kinoshita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takaya","family":"Tamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikako","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Honjo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970110807"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000263699"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19960901)25:1<37::AID-CYTO5>3.0.CO;2-B"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1399-0004.1990.tb03545.x","article-title":"Ratio of fetal to maternal DNA is less than 1 in 5000 at different gestational ages in maternal blood","volume":"98","author":"G\u00e4nshirt\u2010Ahlert D","year":"1990","journal-title":"Clin Genet"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7292(03)00124-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00217766"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0897.2003.01165.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0223(200101)21:1<14::AID-PD970>3.0.CO;2-#"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00217445"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(98)00072-7"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980701)32:3<178::AID-CYTO3>3.0.CO;2-G"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(91)90024-L"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0223(200103)21:3<165::AID-PD29>3.0.CO;2-F"},{"key":"e_1_2_1_15_1","volume-title":"Pathologisch\u2010anatomische Untersuchungen \u00fcber Puerperal Eklampsie","author":"Schmorl G","year":"1893"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0223(200011)20:11<886::AID-PD942>3.0.CO;2-4"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0223(200101)21:1<31::AID-PD974>3.0.CO;2-L"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00214186"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000263950"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0223(199708)17:8<743::AID-PD144>3.0.CO;2-3"},{"key":"e_1_2_1_21_1","first-page":"1089","volume-title":"Internal Medicine","author":"Steinberg MH","year":"1990"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970150116"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.12.1162"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0223(199805)18:5<455::AID-PD309>3.0.CO;2-T"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(69)91642-0"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.030064"},{"key":"e_1_2_1_27_1","first-page":"169","article-title":"Isolation of fetal nucleated red blood cells from maternal blood","volume":"2","author":"Zou L","year":"2000","journal-title":"J Tongfi Med Univ"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1039","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T23:21:36Z","timestamp":1700263296000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["10.1002\/pd.1039"],"URL":"https:\/\/doi.org\/10.1002\/pd.1039","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11]]}}}