{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:12:09Z","timestamp":1770822729943,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2007,11,12]],"date-time":"2007-11-12T00:00:00Z","timestamp":1194825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2007,12,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To demonstrate a role for microsatellite analysis in the evaluation of prenatal cases with possible placental mesenchymal dysplasia (PMD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We present a case report in which several molecular analyses of amniocytes and products of conception were used in combination with cytogenetic and ultrasound studies to evaluate a pregnancy with a clinical suspicion of PMD. A combination of Southern blotting analysis, methylation\u2010sensitive polymerase chain reaction (PCR) and multiplex ligation\u2010dependent probe amplification (MLPA) was utilized to evaluate methylation patterns in the Beckwith\u2013Wiedemann syndrome (BWS) and Prader\u2013Willi\/Angelman syndrome (PW\/AS) critical regions. A series of microsatellite markers were used to evaluate the possibility of an androgenetic cell line.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Methylation studies performed for the BWS and PW\/AS critical regions were abnormal and consistent with a molecular diagnosis of BWS and Angelman syndrome. Further studies of amniocytes using microsatellite markers identified androgenetic and biparental cell lines in approximately a 10:1 ratio, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Prenatal ultrasonography, karyotyping and molecular genetic evaluation for BWS alone were not sufficient to identify the underlying etiology of PMD in this case. The androgenetic cell line was only identified after microsatellite analysis. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.1879","type":"journal-article","created":{"date-parts":[[2007,11,12]],"date-time":"2007-11-12T11:19:12Z","timestamp":1194866352000},"page":"1238-1244","source":"Crossref","is-referenced-by-count":16,"title":["Utility of microsatellite analysis in evaluation of pregnancies with placental mesenchymal dysplasia"],"prefix":"10.1002","volume":"27","author":[{"given":"Marie N.","family":"Schuetzle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy S.","family":"Uphoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bonnie A.","family":"Hatten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Brian","family":"Dawson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,12]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"214","volume-title":"Genetic Disorders and the Fetus","author":"Benn PA","year":"2004"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.5.467"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0004-8666.2003.00130.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1103"},{"key":"e_1_2_1_6_1","unstructured":"FaulknerN DonnenfeldA Rosenblum\u2010VosL HallamS.2005.Utility of molecular zygosity analysis to determine the genetic composition of a twin pregnancy with an apparent hydatidiform mole. 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