{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:04:22Z","timestamp":1699765462077},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":8813,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1981,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three different cell types of primary amniotic fluid cultures (AF\u2010, F\u2010, and E\u2010cells according to Hoehn <jats:italic>et al.<\/jats:italic>, 1974) were analysed for \u03b1\u2010ketoisocaproate decarboxylation activity by microtechnique. This reaction using L\u2010[1\u2010<jats:sup>14<\/jats:sup>C] leucine as initial substrate is applied for prenatal diagnosis of maple syrup urine disease. Activities of \u03b1\u2010ketoisocaproate decarboxylation did not differ significantly for the respective cell types and comparatively small numbers of cells from any primary amniotic fluid culture can be used for the biochemical assay irrespective of the prevailing cell type.<\/jats:p>","DOI":"10.1002\/pd.1970010402","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T15:36:17Z","timestamp":1172936177000},"page":"235-240","source":"Crossref","is-referenced-by-count":4,"title":["Maple syrup urine disease: \u03b1\u2010Ketoisocaproate decarboxylation activity in different types of cultured amniotic fluid cells"],"prefix":"10.1002","volume":"1","author":[{"given":"U.","family":"Wendel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Gamm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Claussen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)91956-6"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-427150-0.50142-X"},{"key":"e_1_2_1_4_1","first-page":"272","article-title":"Maple syrup urine disease: Branched\u2010chain ketoacid decarboxylation in fibroblasts as measured with amino acids and ketoacids","volume":"29","author":"Dancis J.","year":"1977","journal-title":"Am. 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