{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T22:19:01Z","timestamp":1759702741642},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":8540,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1982,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent technological advances allow the detection and quantitation of subsets of leucocytes using monoclonal antibodies. We have taken advantage of this to study the ontogeny of fetal blood leucocytes, using very small blood samples obtained at fetoscopy. By 14 weeks gestation T cells represent 35 per cent or more of fetal leucocytes and the distribution of the helper\/inducer and suppressor\/cytotoxic subsets is similar to that of adults. B lymphocytes before 161\/2 weeks are low (4\u201320 per cent), but rise to a mean of 28 per cent in 17\u201326 week fetuses. Granulocytic cells, many of which are phenotypically immature, represent 18\u201334 per cent of total leucocytes. The methodology employed is very reliable and offers the opportunity for the prenatal diagnosis of some immunodeficiency disorders, since using the same reagents we have diagnosed children with severe combined immunodeficiency shortly after birth.<\/jats:p>","DOI":"10.1002\/pd.1970020310","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:22:30Z","timestamp":1172964150000},"page":"211-218","source":"Crossref","is-referenced-by-count":38,"title":["Phenotypic analysis of fetal blood leucocytes: Potential for prenatal diagnosis of immunodeficiency disorders"],"prefix":"10.1002","volume":"2","author":[{"given":"David C.","family":"Linch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C. L.","family":"Beverley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland J.","family":"Levinsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles H.","family":"Rodeck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.4049\/jimmunol.126.1.83","article-title":"A monoclonal antibody (BA\u20101) reactive with cells of human B lymphocyte lineage","volume":"126","author":"Abramson C. S.","year":"1981","journal-title":"J. Immunol."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/287332a0"},{"key":"e_1_2_1_4_1","first-page":"87","volume-title":"Proc. XI International Course on Transplant and Clinical Immunology","author":"Beverley P. C. L.","year":"1980"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830110412"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","DOI":"10.4049\/jimmunol.124.4.1943","article-title":"A monoclonal antibody reactive with human peripheral blood monocytes","volume":"124","author":"Breard J.","year":"1980","journal-title":"J. Immunol."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1979.tb00288.x"},{"key":"e_1_2_1_8_1","first-page":"279","article-title":"Ontogenic development of the immune system and effects of fetal growth retardation","volume":"7","author":"Chandra R. K.","year":"1979","journal-title":"J. Perinat. Med."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.1.544"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830071112"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830060713"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830090307"},{"key":"e_1_2_1_13_1","first-page":"561","article-title":"Development of cell\u2010mediated lympholysis in human fetal blood lymphocytes","volume":"42","author":"Rayfield L. S.","year":"1980","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.8.4061"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1980.tb04577.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1976.tb03022.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1542\/peds.64.5.795","article-title":"Ontogeny of human T cells","volume":"64","author":"Stites D. P.","year":"1979","journal-title":"Paediatrics"},{"key":"e_1_2_1_18_1","unstructured":"Thomas R. M. Linch D. C.Identification of leucocyte subsets in the newborn using a variety of monoclonal antibodies: A comparison of preterm full term and small for gestational age infants. Submited for publication."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120115"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1970020310","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1970020310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:38:49Z","timestamp":1699767529000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1970020310"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,7]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1982,7]]}},"alternative-id":["10.1002\/pd.1970020310"],"URL":"https:\/\/doi.org\/10.1002\/pd.1970020310","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,7]]}}}