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A 4\u00b775 mm disc spot was eluted in anti\u2010AFP coated tubes containing 1\u00b70 ml of assay medium. After one hour the medium was vortexed and the tubes washed and counted on a Concept 4<jats:sup>tm<\/jats:sup> (Micro\u2010 medic Systems, Horsham, PA.19044). The sensitivity of the technique is about 9 \u03bcg\/l (35 ng\/l in the assay) by the Rodbard formula. The concordance between the dried blood and the serum RIA tests in normal pregnancies was over 90 per cent at the 95th and 97th percentiles. This assay on dried blood spotted on chromatography paper was tested on 1003 patients and proved to be an ideal alternative to whole serum screening techniques: it minimizes sample manipulations and can easily be integrated into an existing newborn screening programme.<\/jats:p>","DOI":"10.1002\/pd.1970020405","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:25:57Z","timestamp":1172964357000},"page":"265-271","source":"Crossref","is-referenced-by-count":3,"title":["Maternal alphafetoproteln screening by the polypropylene tube immunoradiometric assay on dried blood"],"prefix":"10.1002","volume":"2","author":[{"given":"L.","family":"Dallaire","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Grenier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Ducharme","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. B.","family":"Melancon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Potier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Des Rochers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"299","article-title":"Le Diagnostic Pr\u00e9natal des Maladies G\u00e9n\u00e8tiques au second trimestre de la grossesse, Partie II: Les r\u00e9sultats","volume":"111","author":"Dallaire L.","year":"1982","journal-title":"Union Med. Canada"},{"key":"e_1_2_1_3_1","first-page":"2062","article-title":"Thyroxine (T4) determination in dried blood by radio\u2010 immunoassay: a screening method for neonatal hypothyroidism","volume":"102","author":"Dussault J. H.","year":"1973","journal-title":"Union. Med. 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