{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:28:49Z","timestamp":1756992529381},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":7813,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1984,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adaptations of the techniques of modern molecular biology to prenatal diagnosis has opened new avenues for the detection of genetic diseases. We have taken advantage of the rapid adhesion of colony forming cells in cultured amniotic fluid samples to develop an improved method for molecular diagnosis. By employing the cell adherence regime sickle cell diagnosis using Mst II can be undertaken directly. In addition, hybridization with a cloned repetitive sequence that is of Y origin and has limited autosomal homology permits rapid fetal sexing in 3 to 4 days without compromising conventional cytogenetic or biochemical analysis. This combination of techniques provides a useful adjunct to convential prenatal genetic diagnosis in the second trimester.<\/jats:p>","DOI":"10.1002\/pd.1970040402","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:56:39Z","timestamp":1172966199000},"page":"241-247","source":"Crossref","is-referenced-by-count":12,"title":["Improved direct molecular diagnosis and rapid fetal sexing"],"prefix":"10.1002","volume":"4","author":[{"given":"David I.","family":"Hoar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David B.","family":"Haslam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah M.","family":"Starozik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","unstructured":"Disteche C. Luthy D. Haslam D. B. Hoar D. I.(1984). Prenatal identification of a deleted Y chromosome by cytogenetics and a Y specific repetitive DNA probe.Hum. Genet. in press.","DOI":"10.1007\/BF00273007"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/S0140-6736(78)91629-X","article-title":"Antenatal diagnosis of sickle cell anemia by DNA analysis of aminotic fluild cells","author":"Kan Y. W.","year":"1978","journal-title":"Lancet"},{"key":"e_1_2_1_4_1","first-page":"926","article-title":"Prenatal diagnosis of \u03b2\u2010thalassaemias by amniocentesis. Linkage analysis using polymorphic restriction endonuclease sites","volume":"56","author":"Kazazian H. H.","year":"1980","journal-title":"Blood."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970020107"},{"issue":"2","key":"e_1_2_1_6_1","first-page":"2","article-title":"A compilation of techniques for molecular diagosis and the 'New Genetics'","volume":"1","author":"Hoar D. 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Human Genet."}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1970040402","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1970040402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T23:20:50Z","timestamp":1697757650000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1970040402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,7]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1984,7]]}},"alternative-id":["10.1002\/pd.1970040402"],"URL":"https:\/\/doi.org\/10.1002\/pd.1970040402","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,7]]}}}