{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T11:12:58Z","timestamp":1697886778327},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":7509,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1985,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper describes a sensitive method for the detection of precipitation bands in the qualitative acetylcholinesterase (AChe) gel test. This method\u2013dark field illumination\u2013is compared with two existing methods of detection: epi\u2010illumination of unstained gels and transillumination of dithio\u2010oxamide stained gels. The comparison has been carried out on a selected series of 271 amniotic fluid samples, taken before the 22nd week of gestation. All 96 samples from normal pregnancies were scored as negative with all three visualization methods. For the detection of neural tube defects (NTD) and omphaloceles, dark field illumination proved to be more efficient than epi\u2010illumination or dithio\u2010oxamide staining. In one case of another congenital defect, we obtained a positive result with dark field illumination, and a negative one with the other detection methods. It is concluded that dark field illumination was shown to be the most sensitive method for the detection of precipitation bands.<\/jats:p>","DOI":"10.1002\/pd.1970050313","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T23:58:40Z","timestamp":1172966320000},"page":"237-241","source":"Crossref","is-referenced-by-count":1,"title":["Amniotic fluid gel acetylcholinesterase determination in prenatal diagnosis: Dark field illumination as a method for improving the detection of precipitation bands"],"prefix":"10.1002","volume":"5","author":[{"given":"N. J.","family":"Leschot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Heyting","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"Schaik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. J. W.","family":"Redeker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970040503"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(82)90415-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320120314"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/0002-9378(83)90717-2","article-title":"Densitometry as an aid in amniotic fluid gel acetylcholinesterase analysis","volume":"145","author":"Goldfine C.","year":"1983","journal-title":"Amer. J. Obstet. Gynecol."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1978.tb15625.x"},{"key":"e_1_2_1_7_1","first-page":"1","article-title":"Prenatal diagnosis of open neural tube defects using the amniotic fluid acetylcholinesterase assay","volume":"59","author":"Milunsky A.","year":"1982","journal-title":"Obstet. Gynaecol."},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1982.tb04675.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(79)91144-9"},{"key":"e_1_2_1_10_1","first-page":"321","article-title":"Amniotic fluid acetylcholinesterase electrophoresis as a secondary test in the diagnosis of anencephaly and spina bifida in early pregnancy","author":"Wald N. J.","year":"1981","journal-title":"Lancet"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970040502"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1970050313","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1970050313","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T13:03:20Z","timestamp":1697807000000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1970050313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,5]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1985,5]]}},"alternative-id":["10.1002\/pd.1970050313"],"URL":"https:\/\/doi.org\/10.1002\/pd.1970050313","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,5]]}}}