{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T21:41:21Z","timestamp":1698010881182},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":7264,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper describes a method for biochemical analysis of proteins from fetal skin biopsy samples. The method has wide potential application for diagnosis of disorders with a known protein abnormality detectable by protein staining or a specific antibody. Analysis requires a single 1 mm biopsy, is rapid (2 days) and extremely sensitive. In the present study, fetal skin biopsies from normal fetuses and a fetus at risk for lamellar ichthyosis were obtained. The epidermis or hairs with attached follicular cells were dissected from the remaining skin. Proteins were extracted and separated by SDS\u2010polyacrylamide gel electrophoresis. Proteins from duplicate gels were transferred to nitrocellulose and immunostained for the acidic and basic keratins and for the keratin filament associated protein, filaggrin, using monoclonal antibodies. All samples contained keratins typical of fetal epidermis at 20 weeks gestation. Presence of filaggrin is variable at this age and depends on the presence of keratinized cells of hair canals. No keratin abnormalities in the fetus at risk for lamellar ichthyosis were detected, however, in one presumably normal biopsy, an abnormally low proportion of the 67 kd keratin and the presence of follicular keratins were evident. These results demonstrate that biochemical analysis of fetal biopsies is possible, thus increasing the diagnostic potential of the fetal biopsy procedure for disorders in which a known protein or antigen is altered <jats:italic>in utero<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/pd.1970060106","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T19:21:35Z","timestamp":1172949695000},"page":"37-44","source":"Crossref","is-referenced-by-count":4,"title":["Biochemical examination of fetal skin biopsy specimens obtained by fetoscopy: Use of the method for analysis of keratins and filaggrin"],"prefix":"10.1002","volume":"6","author":[{"given":"Beverly A.","family":"Dale","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy B.","family":"Perry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen A.","family":"Holbrook","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily F.","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vyta","family":"Senikas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.4.1257"},{"key":"e_1_2_1_3_1","first-page":"321","article-title":"Characterization of two monoclonal antibodies to human epidermal keratohyalin","volume":"84","author":"Dale B. A.","year":"1985","journal-title":"J. Invest. Dermatol."},{"key":"e_1_2_1_4_1","first-page":"549","article-title":"Properties of hair keratin in an autosomal dominant form of ectodermal dysplasia","volume":"24","author":"Gold R. J. M.","year":"1972","journal-title":"Amer. J. Hum. Genet."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(82)90085-7"},{"key":"e_1_2_1_6_1","first-page":"155","article-title":"Prenatal diagnosis of genetic skin disease","volume":"3","year":"1984","journal-title":"Seminars in Dermatology"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12556818"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12534504"},{"key":"e_1_2_1_9_1","unstructured":"Holbrook K. A. Dale B. A. Perry T. B. Fisher C. Hamilton E. F. Hoff M. S. Senikas V. Sybert V. P. Phenotypic expression of lamellar ichthyosis in second trimester fetal skin. J. Invest. Dermatol (submitted)."},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of the bacteriophage T4","volume":"226","author":"Laemmli U. K.","year":"1970","journal-title":"Nature"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1982.tb01280.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00694618"},{"key":"e_1_2_1_13_1","unstructured":"PerryT. B. HolbrookK. A. HoffM. S. HamiltonE. F. SenikasV. FisherC. Prenatal diagnosis of congenital non\u2010bullous ichthyosiform erythroderma (lamellar ichythyosis). Prenat. diagn. (submitted)."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(78)90005-3"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12540831"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12264813"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_18_1","unstructured":"WittD. R. HaydenM. R. HolbrookK. A. DaleB. A. BaldwinV. TaylorG. P. Restrictive dermopathy: a newly recognized autosomal recessive skin dysplasia.Am. J. Med. Genet. in press."}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1970060106","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1970060106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T23:53:40Z","timestamp":1697932420000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1970060106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/pd.1970060106"],"URL":"https:\/\/doi.org\/10.1002\/pd.1970060106","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}