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Adequate samples were obtained in 99\u00b71 per cent and in all these cases the fetal karyotype was established. A chromosome abnormality was found in 2\u00b70 per cent of cases. A discrepancy between the karyotype obtained after 24 h incubation and the karyotype in cell culture was observed in 2\u00b73 per cent. Maternal cell contamination in the cultures was confirmed in 13 of 181 cases where the 24 h incubation revealed a male karyotype. Studies of culture morphology showed that colonies of convoluted cells may serve as a marker for contamination with maternal cells in culture. For the present, we recommend using a short\u2010term method as well as cell culture for cytogenetic investigation until the problems with karyotype discrepancy and maternal cell contamination have been further clarified.<\/jats:p>","DOI":"10.1002\/pd.1970080104","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T01:31:08Z","timestamp":1172971868000},"page":"19-31","source":"Crossref","is-referenced-by-count":15,"title":["Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester"],"prefix":"10.1002","volume":"8","author":[{"given":"A. J.","family":"Therkelsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. K. A.","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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