{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T08:20:44Z","timestamp":1759566044330},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":5834,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1989,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hypoxanthine phosphoribosyl transferase (HPRT) and adenine phosphoribosyl transferase (APRT) activities in individual non\u2010fertilized human eggs and in human pre\u2010embryos (4\u2010cell to blastocyst stage) have been analysed. A wide spread of activities was observed, the mean values of which decline with time post\u2010ovulation for both eggs and advancing preembryonic stages. The variation in activities was less in groups of eggs or pre\u2010embryos recovered from a single ovulatory cycle. The activity of HPRT, but not of APRT, was readily detectable in single 4\u2010cell and 8\u2010cell blastomeres. When pre\u2010embryos at various preimplantation stages were exposed to \u03b1\u2010amanitin, to block transcription of mRNA from the preembryonic genome, no clear effect on HPRT activity was observed. It is concluded that the HPRT and APRT activities measured in the pre\u2010embryos studied here are likely to be maternally inherited, and that use of a direct assay for HPRT activity for the pre\u2010implantation diagnosis of Lesch\u2013Nyhan disease would be premature.<\/jats:p>","DOI":"10.1002\/pd.1970091204","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T22:10:10Z","timestamp":1172959810000},"page":"839-850","source":"Crossref","is-referenced-by-count":23,"title":["Measurement of HPRT activity in the human unfertilized oocyte and pre\u2010embryo"],"prefix":"10.1002","volume":"9","author":[{"given":"Peter R.","family":"Braude","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilyn","family":"Monk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan J.","family":"Pickering","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Cant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin H.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1093\/oxfordjournals.humrep.a136813","article-title":"Microassay for adenosine deaminase, the enzyme lacking in some forms of immunodeficiency, in mouse preimplantation embryos","volume":"3","author":"Benson C.","year":"1988","journal-title":"Hum. 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