{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T06:11:26Z","timestamp":1698041486711},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":5713,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thirty\u2010six pregnancies in 25 families at risk of \u03b2\u2010thalassaemia major received prenatal diagnosis. Chorionic villus sampling or amniocentesis was done in 35 pregnancies to obtain fetal cells for DNA linkage study, for which Southern blotting and DNA hybridization were used to detect seven restriction fragment length polymorphisms (RFLPs) within the \u03b2\u2010globin gene cluster: \u03f5\u2010HincII, <jats:sup>G<\/jats:sup>\u03b3\u2010HindIII, <jats:sup>A<\/jats:sup>\u03b3\u2010HindIII, \u03a6\u03b2\u2010HincII, 3\u2032\u03a6\u03b2\u2010HincII, \u03b2\u2010AvaII, and 3\u2032\u03b2 BarnHI. \u03b2\u2010Thalassaemia major was diagnosed in seven and excluded in 22 pregnancies. In the remaining six cases, \u03b2\u2010thalassaemia major could not be excluded. In these six pregnancies and another one with late booking, ultrasound\u2010guided cordocentesis was performed at the 22nd to 27th week of gestation. Globin chain composition was determined with urea\u2010acetic acid\u2010Triton X\u2010100\u201012 per cent polyacrylamide gel electrophoresis. \u03b2\u2010Thalassaemia major was diagnosed in two fetuses and excluded in the other five. Eleven fetuses (in which \u03b2\u2010thalassaemia major was excluded) have been delivered and are healthy at more than 5 months old DNA linkage analysis coupled with globin chain electrophoresis provides an effective way for prenatal diagnosis of \u03b2\u2010thalassaemia major, although these methods are being replaced by more direct detection techniques using oligonucleotide probes.<\/jats:p>","DOI":"10.1002\/pd.1970100405","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T03:55:56Z","timestamp":1172980556000},"page":"237-244","source":"Crossref","is-referenced-by-count":3,"title":["DNA polymorphism and globin chain analysis in the prenatal diagnosis of \u03b2\u2010thalassaemia major in Taiwan"],"prefix":"10.1002","volume":"10","author":[{"given":"Tsang\u2010Ming","family":"Ko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fon\u2010Jou","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu\u2010Yao","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630268108991810"},{"key":"e_1_2_1_3_1","first-page":"1597","volume-title":"Hematology","author":"Atwater J.","year":"1977"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1182\/blood.V71.5.1357.1357","article-title":"A simple approach to prenatal diagnosis of \u03b2\u2010halassemia in a geographic area where multiple mutations occur","volume":"71","author":"Cai S. 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