{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T05:02:21Z","timestamp":1698382941791},"reference-count":13,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":5683,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1990,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exclusion of \u03b2\u2010thalassaemia in mice was undertaken by pre\u2010implantation DNA diagnosis. Biopsies of approximately five cells were obtained from mouse pre\u2010embryos. DNA prepared from this material was amplified by the polymerase chain reaction (PCR), enabling identification of the normal \u03b2\u2010major globin gene in 11 out of 20 cases where it was known to be present. The expected negative result was obtained in 14 out of 16 homozygous thalassaemic embryos, with two false positives. Results are promptly available following PCR, thereby facilitating re\u2010implantation of embryos which have had homozygous \u03b2\u2010thalassaemia excluded. The mouse model illustrates a useful approach to pre\u2010implantation diagnosis of genetic disease in the human.<\/jats:p>","DOI":"10.1002\/pd.1970100504","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T03:54:21Z","timestamp":1172980461000},"page":"295-301","source":"Crossref","is-referenced-by-count":4,"title":["Exclusion of \u03b2\u2010thalassaemia by biopsy and DNA amplification in mouse pre\u2010embryos"],"prefix":"10.1002","volume":"10","author":[{"given":"R.","family":"Lindeman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Lutjen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"O'Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Trent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"5169","article-title":"Early detection of genetic and biochemical disorders: preimplantation diagnosis","volume":"28","author":"Brown N. A.","year":"1988","journal-title":"Congenital Anomalies"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"12368","DOI":"10.1016\/S0021-9258(18)67249-7","article-title":"A 66\u2010base pair insert bridges the deletion responsible for a mouse model of \u03b2\u2010thalassaemia","volume":"261","author":"Goldberg S. Z.","year":"1986","journal-title":"J. Biol Chem."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/332543a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.23.10953"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/335414a0"},{"key":"e_1_2_1_8_1","unstructured":"Lutjen J. Kuzyminska U. O'Neill C.(1989). Viability of mouse embryos following biopsy Proc. VI World Congress on IVF 50."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.66.2.263"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1985.tb37520.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/324163a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2448875"},{"key":"e_1_2_1_13_1","unstructured":"Editor's note. Since this paper was submitted the following article relevant to this investigation was published:"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90655-7"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1970100504","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1970100504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T15:04:29Z","timestamp":1697987069000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1970100504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,5]]},"references-count":13,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1990,5]]}},"alternative-id":["10.1002\/pd.1970100504"],"URL":"https:\/\/doi.org\/10.1002\/pd.1970100504","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,5]]}}}