{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T18:47:48Z","timestamp":1785350868569,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":5165,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1991,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast\u2010like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y\u2010specific sequences from mothers carrying male fetuses. At the ultra\u2010structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter\u2010villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y\u2010specific sequences in two out of three samples of sorted TLCs.<\/jats:p><jats:p>These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non\u2010invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.<\/jats:p>","DOI":"10.1002\/pd.1970111007","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T04:15:41Z","timestamp":1172981741000},"page":"787-798","source":"Crossref","is-referenced-by-count":42,"title":["Trophoblast\u2010like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification"],"prefix":"10.1002","volume":"11","author":[{"given":"J. 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