{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:46:50Z","timestamp":1759942010162},"reference-count":9,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":4556,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1993,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated the ability of high\u2010resolution proton NMR spectroscopy to provide a biochemical constituent screening of human amniotic fluid (AF). Proton NMR spectra were obtained at 300 MHz on AF from patients undergoing amniocentesis in the mid\u2010trimester. Only AF from normal pregnancies (normal fetal karyotype, normal a\u2010fetoprotein levels, normal birth outcome) was used in this study. The AF supernatant was lyophilized and resuspended in deuterated water containing 0.1 mm phosphate buffer and 6.02 mm disodium maleate. Identification of low molecular weight compounds was confirmed by two\u2010dimensional NMR spectra (primarily correlated spectroscopy, or COSY) and standard addition techniques. A broad profile of compounds were \u2018NMR visible\u2019 in a single proton spectrum, including creatinine, glucose, organic acids (acetate, citrate, and lactate) and several amino acids (alanine, histidine, leucine, phenylalanine, tyrosine and valine). The proton spectrum was unaffected by prior freezing\/thawing of AF samples. We were able to quantify compounds by comparison with an added concentration standard (maleate) at concentrations as low as 30 \u03bcm. Good agreement with literature values based on other analytical techniques was obtained.<\/jats:p>","DOI":"10.1002\/pd.1970130609","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T06:49:10Z","timestamp":1172990950000},"page":"473-480","source":"Crossref","is-referenced-by-count":21,"title":["Quantification of human amniotic fluid constituents by high resolution proton nuclear magnetic resonance (NMR) spectroscopy"],"prefix":"10.1002","volume":"13","author":[{"given":"Cynthia J.","family":"Sims","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don T.","family":"Fujito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis R.","family":"Burholt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Dadok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harlan R.","family":"Giles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Allan","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1940020513"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(73)91603-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(72)90402-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970070511"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6565(89)80008-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910210113"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4612-3300-8","volume-title":"NMR: Principles and Applications to Biomedical Research","author":"Pettegrew J. W.","year":"1990"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0002-9378(71)90898-2","article-title":"Quantitative amino acids in amniotic fluid and maternal plasma in early and late pregnancy","volume":"11","author":"Reid D. W. J.","year":"1971","journal-title":"Am. J. Obstet. Gynecol."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)91106-M"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.1970130609","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.1970130609","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T15:47:07Z","timestamp":1698248827000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.1970130609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,6]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1993,6]]}},"alternative-id":["10.1002\/pd.1970130609"],"URL":"https:\/\/doi.org\/10.1002\/pd.1970130609","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,6]]}}}