{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T09:11:54Z","timestamp":1698311514723},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,21]],"date-time":"2005-11-21T00:00:00Z","timestamp":1132531200000},"content-version":"vor","delay-in-days":4373,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid karyotyping in the second and third trimesters has important implications for the management of pregnancies at risk. From September 1985 to March 1992, 735 amniotic fluid samples sent to our laboratory for rapid karyotyping from 64 different diagnostic centres of the Federal Republic of Germany were included in a comparative study on harvesting for chromosome analysis using the \u2018pipette method\u2019 or the \u2018<jats:italic>in situ<\/jats:italic>\u2019 technique. The average time between preparation of the amniotic fluid and verbal notification of the analysed karyotype was 5\u00b741 days. The \u2018pipette method\u2019 needed on average 4\u00b765 days, and the \u2018<jats:italic>in situ<\/jats:italic>\u2019 technique 5\u00b797 days. In comparison with other more invasive techniques available for rapid karyotyping such as cordocentesis and placental biopsy, amniocentesis and subsequent chromosome harvesting using the \u2018pipette method\u2019 and\/or the \u2018<jats:italic>in situ<\/jats:italic>\u2019 technique proved very useful and efficient. The overall incidence of chromosome aberrations was 15\u00b73 per cent. The high rate of structural chromosome aberrations and uncommon aneuploidies found in our investigation (12 per cent) indicates that for rapid karyotyping in the second and third trimesters, conventional cytogenetic techniques cannot be replaced by faster techniques based on fluorescent <jats:italic>in situ<\/jats:italic> hybridization on interphase cells in the near future.<\/jats:p>","DOI":"10.1002\/pd.1970131203","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T06:39:44Z","timestamp":1172990384000},"page":"1085-1093","source":"Crossref","is-referenced-by-count":4,"title":["Rapid karyotyping in prenatal diagnosis: A comparative study of the \u2018pipette method\u2019 and the \u2018<i>in situ<\/i>\u2019 technique for chromosome harvesting"],"prefix":"10.1002","volume":"13","author":[{"given":"Uwe","family":"Claussen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renate","family":"Ulmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernst","family":"Beinder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans\u2010Joachim","family":"Voigt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970080413"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00220460"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00278985"},{"key":"e_1_2_1_5_1","first-page":"33","article-title":"Die 'Pipettenmethode' zur schnellen Karyotypisierung bei sonographischem Verdacht f\u00fcr eine Chromosomenanomalie","volume":"17","author":"Claussen U.","year":"1984","journal-title":"Gyn\u00e4kologe"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00270553"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970060603"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.1992.tb03642.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.280.6222.1107"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970030402"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(85)80254-4"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970090405"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1985.tb03070.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)93293-V"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970090902"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)90014-X"},{"key":"e_1_2_1_17_1","volume-title":"An International System for Human Cytogenetic Nomenclature","author":"ISCN 1985","year":"1985"},{"key":"e_1_2_1_18_1","first-page":"338","article-title":"Placental mosaicism and intrauterine survival of trisomies 13 and 18","volume":"44","author":"Kalousek D. 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