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Optimal conditions for the assay and the ranges of enzyme activity were established for the various types of fetal cells. This simple fluorometric assay provides a reliable method for early prenatal diagnosis of Sanfilippo disease type C which is more convenient than current methods using radiolabelled substrates. The method was applied to amniotic fluid cells and fetal fibroblasts from an at\u2010risk pregnancy in which an affected fetus was diagnosed by two\u2010dimensional electrophoresis of glycosaminoglycans in the amniotic fluid.<\/jats:p>","DOI":"10.1002\/pd.1970140104","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T06:51:42Z","timestamp":1172991102000},"page":"17-22","source":"Crossref","is-referenced-by-count":20,"title":["Prenatal diagnosis of sanfilippo disease type C using a simple fluorometric enzyme assay"],"prefix":"10.1002","volume":"14","author":[{"given":"Wang","family":"He","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya. V.","family":"Voznyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. G. M.","family":"Huijmans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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