{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:25:47Z","timestamp":1779344747935,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,12,14]],"date-time":"2005-12-14T00:00:00Z","timestamp":1134518400000},"content-version":"vor","delay-in-days":3696,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[1995,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Herlitz junctional epidermolysis bullosa (HJEB) is a severe blistering disorder which usually results in death during infancy. We have previously shown that the anchoring filament protein laminin\u20105 (kalinin\/nicein), which mediates keratinocyte attachment and dermal\u2013epidermal cohesion, is abnormally expressed in individuals with HJEB. Laminin\u20105 was detected by Western blot analysis in amniotic fluid from 44 consecutive normal secondtrimester control pregnancies, but was undetectable in second\u2010trimester amniotic fluid from four pregnancies with fetuses affected by HJEB. In one case of severe non\u2010Herlitz JEB, laminin\u20105 was detected in both amniotic fluid and skin. In human amniotic fluid, the laminin\u20105 a3 subunit was processed to a major 165 kD species and a minor 145 kD species and the \u03b22 subunit was partially processed to 105 kD. Although laminin\u20105 was covalently associated with laminin\u20106 (K\u2010laminin) in amniotic membrane, no covalent interaction was detected in amniotic fluid. Laminin\u20105 from amniotic fluid strongly supported keratinocyte attachment. These results suggest that Western blot analysis of second\u2010trimester amniotic fluid is useful in determining the prenatal diagnosis of HJEB and that laminin\u20105 may serve a physiologically important function in amniotic fluid.<\/jats:p>","DOI":"10.1002\/pd.1970151107","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T10:10:50Z","timestamp":1173003050000},"page":"1027-1034","source":"Crossref","is-referenced-by-count":8,"title":["Prenatal diagnosis of herlitz junctional epidermolysis bullosa by amniocentesis"],"prefix":"10.1002","volume":"15","author":[{"given":"M. Peter","family":"Marinkovich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guerrino","family":"Meneguzzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Burgeson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudine","family":"Blanchet\u2010Bardon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen A.","family":"Holbrook","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynne T.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela M.","family":"Christiano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Paul","family":"Ortonne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0394-299"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117041"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0945-053X(94)90184-8"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(91)70021-S"},{"key":"e_1_2_1_6_1","first-page":"1","volume-title":"Epidermolysis Bullosa: A Clinical, Genetic and Epidemiologic Study","author":"Gedde\u2010Dahl T.","year":"1971"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-775170-2.50018-4"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1001\/archderm.1988.01670050070024","article-title":"Extracutaneous epithelial involvement in inherited epidermolysis bullosa","volume":"124","author":"Holbrook K. 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