{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:16:04Z","timestamp":1775765764078,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,11,13]],"date-time":"2008-11-13T00:00:00Z","timestamp":1226534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hubei Province Key Technologies Program","award":["2006AA301C21"],"award-info":[{"award-number":["2006AA301C21"]}]},{"name":"Hubei Province Family Planning Research Project","award":["KG2006-2"],"award-info":[{"award-number":["KG2006-2"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine if quantitative abnormalities of circulating fetal trophoblast cells (CFTCs) are associated with preeclampsia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The trophoblast cell\u2010specific antibody, MEM\u2010G\/9 (monoclonal antibody to HLA\u2010G), was applied to distinguish the trophoblast cells from the maternal circulation. The trophoblast cells were isolated by density\u2010gradient centrifugation from maternal blood samples of normal pregnant and preeclamptic women, respectively. After preliminary enrichment, the CFTCs were identified by immunocytochemical staining with the MEM\u2010G\/9. To prove fetal origin of the HLA\u2010G\u2010positive cells, primer\u2010extension preamplification (PEP) and polymerase chain reaction (PCR) based on single HLA\u2010G\u2010positive cells were adopted to detect human sex\u2010determining region of the Y\u2010chromosome (SRY) gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There were 6.88 \u00b1 1.54 and 30.56 \u00b1 5.16 HLA\u2010G\u2010positive cells in 6 mL maternal blood from the normal pregnant (n = 16) and preeclamptic women (n = 18), respectively. The difference was statistically significant (<jats:italic>p<\/jats:italic> &lt; 0.001). The SRY gene from the HLA\u2010G\u2010positive cells was detected in all pregnant women carrying male fetuses. The sensitivity and specificity of PEP and PCR for the SRY gene detection were 100%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It is concluded that enhancement of CFTCs numbers is related to preeclampsia, which provides information useful for noninvasive prenatal diagnosis of preeclampsia. 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