{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:13:02Z","timestamp":1788869582865,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T00:00:00Z","timestamp":1229558400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"AF"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\u03b2\u2010Human chorionic gonadotropin (HCG) and pregnancy\u2010associated plasma protein (PAPP\u2010A) are placentally produced proteins whose levels are altered in pregnancies with trisomy 21. PLAC4 is located on chromosome 21 and its expression is restricted to the placenta. Here we investigated whether the levels of \u03b2\u2010HCG\u2010, PAPP\u2010A\u2010 and PLAC4 mRNA could be able to discriminate pregnancies whose fetus is affected by trisomy 21.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>Hundred and forty\u2010three blood samples from normal pregnancies and eight samples from trisomic pregnancies were collected. Total RNA was extracted from whole maternal blood, reverse\u2010transcribed and the three mRNAs were quantified by real\u2010time quantitative PCR. Hundred and nine controls were also tested for the serum levels of PAPP\u2010A and HCG proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\u03b2\u2010HCG and PLAC4 mRNAs were detected in all samples, in higher amounts than in plasma, whereas the detection rate for PAPP\u2010A mRNA was below 10%. The levels of \u03b2\u2010HCG mRNA significantly correlated with the circulatory concentrations of the HCG protein. However, neither \u03b2\u2010HCG\u2010 nor PLAC4 mRNAs show a significant difference between cases and controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Maternal blood levels of \u03b2\u2010HCG\u2010, PLAC4\u2010 and PAPP\u2010A mRNAs are not useful markers for the screening of pregnancies with trisomy 21 as their concentrations are either not significantly altered (\u03b2\u2010HCG and PLAC4) or too low to be detected (PAPP\u2010A). Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.2163","type":"journal-article","created":{"date-parts":[[2008,12,19]],"date-time":"2008-12-19T13:25:28Z","timestamp":1229693128000},"page":"1262-1267","source":"Crossref","is-referenced-by-count":5,"title":["PLAC4 and \u03b2\u2010HCG mRNA levels are not altered in the maternal circulation of pregnancies with trisomy 21"],"prefix":"10.1002","volume":"28","author":[{"given":"Irina","family":"Banzola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Rusterholz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Letizia","family":"Zannoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Rizzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao 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