{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:33:53Z","timestamp":1758926033453},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T00:00:00Z","timestamp":1272931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"European Union FP6","award":["0.7244"],"award-info":[{"award-number":["0.7244"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Maternal serum A Disintergrin And Metaloprotease\u201012 (ADAM\u201012) has been proposed as a marker for prenatal screening of chromosomal abnormalities, pre\u2010eclampsia and other adverse pregnancy outcomes. In this study, we examine the stability of ADAM\u201012 with time and at different temperatures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Maternal serum and whole blood pools were stored at 30 \u00b0C, room temperature and refrigerator temperature or subjected to repeated freeze\u2013thaw cycles. ADAM\u201012 was measured at set time points using an automated DELFIA\u00ae research assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using a 10% change in concentration as a limit of stability, ADAM\u201012 is stable in serum for less than 15 h at 30 \u00b0C, less than 20 h at room temperature and for 51 h at refrigerator temperature. ADAM\u201012 levels are not altered following three \u2212 20 \u00b0C to room temperature freeze\u2010thaw cycles. The stability of ADAM\u201012 in whole blood appears similar to that in serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The findings of this study suggest that ADAM\u201012 may be unstable under many routine laboratory conditions, and the marker's instability may also be partly responsible for the discrepancies in the literature. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.2522","type":"journal-article","created":{"date-parts":[[2010,5,5]],"date-time":"2010-05-05T02:53:18Z","timestamp":1273027998000},"page":"555-560","source":"Crossref","is-referenced-by-count":7,"title":["ADAM\u201012 stability in first trimester maternal serum"],"prefix":"10.1002","volume":"30","author":[{"given":"N. J.","family":"Cowans","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Stamatopoulou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Jaakohuhta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Spencer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,5,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1093\/clinchem\/37.3.398","article-title":"Determination of product shelf life and activation energy for five drugs of abuse","volume":"37","author":"Anderson G","year":"1991","journal-title":"Clin Chem"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2405"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1750"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1665"},{"key":"e_1_2_1_6_1","article-title":"PAPP\u2010A and free beta\u2010hCG stability in first trimester serum using PerkinElmer AutoDELFIA and DELFIA Xpress systems","author":"Cowans NJ","year":"2009","journal-title":"Prenat Diagn"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2043"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.1.157"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1029"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1480"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.762"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000165829.65319.65"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1540"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1326"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3835"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2141"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e318188d6f9"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.12.029"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409565200"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002172200"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767050701483389"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1885"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1957"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1994"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1978"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1939"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767050902755478"},{"key":"e_1_2_1_28_1","first-page":"123","volume-title":"The ADAM Family of Proteases","author":"Wewer UM","year":"2006"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2300"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.2522","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.2522","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.2522","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T10:41:50Z","timestamp":1700649710000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.2522"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,21]]},"references-count":28,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1002\/pd.2522"],"URL":"https:\/\/doi.org\/10.1002\/pd.2522","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,21]]}}}