{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:33:06Z","timestamp":1788870786319,"version":"build-2803163510"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T00:00:00Z","timestamp":1272931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Family Planning Committee of Zhejiang Province","award":["2008-87"],"award-info":[{"award-number":["2008-87"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To estimate the use of maternal serum ADAM12 as a second\u2010trimester Down syndrome serum marker.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Samples from a total of 46 Down syndrome pregnancies and 184 unaffected singleton pregnancies matched for gestational age and maternal weight were retrieved from storage and measured for ADAM12; 35 false\u2010positive pregnancies were included among the controls to assess reductions in false\u2010positive rates by inclusion of ADAM12 in the risk calculation of an algorithm that used \u03b1\u2010fetoprotein (AFP) and human chorionic gonadotrophin (hCG) (double screen).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ADAM12 was measured and expressed as multiple of the gestation\u2010specific median (MoM) and corrected for maternal weight. The median ADAM12 level in the affected pregnancies was 1.26 MoM compared with 1.0 MoM in the unaffected control pregnancies (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). In unaffected pregnancies, there was a significant correlation between ADAM12 and AFP (\n                      <jats:italic>r<\/jats:italic>\n                      = 0.314) but not hCG (\n                      <jats:italic>r<\/jats:italic>\n                      = 0.018). Statistical modeling predicted that ADAM12 as a second serum marker could increase the detection rate from 48 to 85%, while reducing the false\u2010negative and false\u2010positive rates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>ADAM12 can be used as an effective second\u2010trimester serum marker for prenatal screening of Down syndrome. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pd.2523","type":"journal-article","created":{"date-parts":[[2010,5,4]],"date-time":"2010-05-04T18:16:56Z","timestamp":1272997016000},"page":"561-564","source":"Crossref","is-referenced-by-count":7,"title":["ADAM12 is an effective marker in the second trimester of pregnancy for prenatal screening of Down syndrome"],"prefix":"10.1002","volume":"30","author":[{"given":"Min","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiming","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuning","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1750"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/096914139500200410"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1665"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.1.157"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1258\/0004563021901559"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.4997"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1540"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1599"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3835"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1093\/clinchem\/42.8.1196","article-title":"One\u2010step all\u2010in\u2010one dry reagent immunoassays with fluorescent europium chelate label and time\u2010resolved fluorometry","volume":"42","author":"L\u00f6vgren T","year":"1996","journal-title":"Clin Chem"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000278820.54029.e3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2005.034280"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10038-007-0229-z"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002172200"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.309.6957.776"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1999.tb08183.x"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.2523","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.2523","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.2523","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T05:41:36Z","timestamp":1700631696000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.2523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,21]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1002\/pd.2523"],"URL":"https:\/\/doi.org\/10.1002\/pd.2523","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,21]]}}}