{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T20:56:10Z","timestamp":1768337770682,"version":"3.49.0"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T00:00:00Z","timestamp":1352332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Chromosome analysis is the traditional method for detecting genetic abnormalities in products of conception, but it is prone to a high failure rate because of the requirement for cell culture. Molecular genetic tests do not require cell culture, but are either more expensive (e.g. chromosomal microarray) or less sensitive than chromosome analysis (e.g. fluorescence <jats:italic>in situ<\/jats:italic> hybridization, multiplex ligation mediated amplification). The KaryoLite\u2122 BACs\u2010on\u2010Beads\u2122 (KL\u2010BoBs\u2122) assay is highly multiplexed with low resolution coverage and is designed to detect aneusomy for any chromosome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively tested 100 products of conception samples previously characterized by karyotype (<jats:italic>n<\/jats:italic>\u2009=\u200990), and\/or microarray (<jats:italic>n<\/jats:italic>\u2009=\u200961) using KL\u2010BoBs\u2122. We included samples extracted from either cultured or direct specimens from placental villi or fetal somatic tissue, with a variety of chromosomal abnormalities typically identified in our clinical cytogenetics laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>KL\u2010BoBs\u2122 and microarray results were concordant for all cases of aneusomy. On the basis of a review of 3794 consecutive cases in our laboratory, aneusomy accounts for 74.3% of abnormalities detected. Polyploidy and structural abnormalities were not detected by KL\u2010BoBs\u2122.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>KL\u2010BoBs\u2122 is potentially very useful as a first line test for aneusomy detection because of its lower cost, rapid detection, and ability to generate a molecular karyotype for samples that fail to grow in culture. \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.4003","type":"journal-article","created":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T06:56:04Z","timestamp":1352444164000},"page":"25-31","source":"Crossref","is-referenced-by-count":10,"title":["Rapid aneusomy detection in products of conception using the KaryoLite\u2122 BACs\u2010on\u2010Beads\u2122 assay"],"prefix":"10.1002","volume":"33","author":[{"given":"Christian N.","family":"Paxton","sequence":"first","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology\u00ae ARUP Laboratories  Salt Lake City UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur R.","family":"Brothman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Utah School of Medicine  Salt Lake City UT USA"},{"name":"Department of Human Genetics University of Utah School of Medicine  Salt Lake City UT USA"},{"name":"Department of Pathology University of Utah School of Medicine  Salt Lake City UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine B.","family":"Geiersbach","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Utah School of Medicine  Salt Lake City UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/GRF.0b013e318161719c"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1385\/1592598331"},{"key":"e_1_2_7_4_1","volume-title":"Chromosome Abnormalities and Genetic Counseling","author":"Sutherland GA","year":"2012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.GIM.0000160075.96707.04"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e3181abc92a"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)01801-X"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181bc6ab0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.3825"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1411"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e3181faa0d2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1777"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/GIM.0b013e31820c685b"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2424"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2727"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2766"},{"key":"e_1_2_7_17_1","first-page":"1","article-title":"Cytogenetics of pregnancy wastage","volume":"14","author":"Boue A","year":"1985","journal-title":"Adv Hum Genet"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2005.06.023"}],"container-title":["Prenatal Diagnosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.4003","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpd.4003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pd.4003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T09:12:01Z","timestamp":1700644321000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1002\/pd.4003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,8]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1002\/pd.4003"],"URL":"https:\/\/doi.org\/10.1002\/pd.4003","archive":["Portico"],"relation":{},"ISSN":["0197-3851","1097-0223"],"issn-type":[{"value":"0197-3851","type":"print"},{"value":"1097-0223","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,8]]}}}