{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:46:25Z","timestamp":1775065585050,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T00:00:00Z","timestamp":1355961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aims to assess the suitability of non\u2010invasive prenatal <jats:italic>RHD<\/jats:italic> genotyping in non\u2010immunized midtrimester pregnant women from a mixed ethnic population, to prevent unnecessary anti\u2010D immunoglobulin prophylaxis and to identify <jats:italic>RHD<\/jats:italic> variants<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rhesus D\u2010negative pregnant women were offered fetal <jats:italic>RHD<\/jats:italic> genotyping at 24 gestational weeks. A total of 284 samples were tested for <jats:italic>RHD<\/jats:italic> status using multiplex rt\u2010PCR amplification of exons 5 and 7 of the <jats:italic>RHD<\/jats:italic> gene and exons 6 and 10 in selected cases. Women carrying <jats:italic>RHD<\/jats:italic>\u2010negative fetuses were counseled about their option to avoid routine antenatal anti\u2010D immunoglobulin administration. Diagnostic accuracy of <jats:italic>RHD<\/jats:italic> genotyping was compared with postnatal Rhesus D serotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 184 positives (65%), 91 negatives (32%) and 7 cases (2.5%) compatibles with <jats:italic>RHD<\/jats:italic> variants were detected by <jats:italic>RHD<\/jats:italic> genotyping. No false negative results were found, and a single false positive was observed in a twin pregnancy. Genotyping was accepted when offered by 94% of women (284\/302), and anti\u2010D immunoglobulin was avoided in 95% (90\/95) of <jats:italic>RHD<\/jats:italic>\u2010negative fetuses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Non\u2010invasive routine antenatal <jats:italic>RHD<\/jats:italic> genotyping at 24\u2009weeks of pregnancy is a highly accurate method, resulting in the avoidance of 95% of unnecessary administrations of anti\u2010D immunoglobulin, with no false negative results. \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.4035","type":"journal-article","created":{"date-parts":[[2012,12,22]],"date-time":"2012-12-22T04:33:43Z","timestamp":1356150823000},"page":"173-178","source":"Crossref","is-referenced-by-count":19,"title":["Clinical application of midtrimester non\u2010invasive fetal <i>RHD<\/i> genotyping and identification of <i>RHD<\/i> variants in a mixed\u2010ethnic population"],"prefix":"10.1002","volume":"33","author":[{"given":"M.","family":"Grande","sequence":"first","affiliation":[{"name":"Department of Maternal\u2010Fetal Medicine, Institute Gynecology, Obstetrics and Neonatology Hospital Cl\u00ednic Barcelona  Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Ordo\u00f1ez","sequence":"additional","affiliation":[{"name":"Labco\u2010General Lab  Barcelona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Cirigliano","sequence":"additional","affiliation":[{"name":"Labco\u2010General Lab  Barcelona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Cid","sequence":"additional","affiliation":[{"name":"Institut Catal\u00e0 de la Salut  Barcelona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Grau","sequence":"additional","affiliation":[{"name":"Institut Catal\u00e0 de la Salut  Barcelona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Pericot","sequence":"additional","affiliation":[{"name":"Department of Maternal\u2010Fetal Medicine, Institute Gynecology, Obstetrics and Neonatology Hospital Cl\u00ednic Barcelona  Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Teixido","sequence":"additional","affiliation":[{"name":"Department of Maternal\u2010Fetal Medicine, Institute Gynecology, Obstetrics and Neonatology Hospital Cl\u00ednic Barcelona  Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Marin","sequence":"additional","affiliation":[{"name":"Centre de Diagnostic Biomedic  Barcelona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Borrell","sequence":"additional","affiliation":[{"name":"Department of Maternal\u2010Fetal Medicine, Institute Gynecology, Obstetrics and Neonatology Hospital Cl\u00ednic Barcelona  Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31817d453c"},{"key":"e_1_2_6_3_1","unstructured":"Routine antenatal anti\u2010D prophylaxis for women who are rhesus D negative. Review of NICE technology appraisal guidance 41. 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