{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T02:52:28Z","timestamp":1771296748725,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,27]],"date-time":"2013-01-27T00:00:00Z","timestamp":1359244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to characterize placenta\u2010specific microRNAs in fetal growth restriction (FGR) pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Placenta\u2010specific miRNAs were identified by next\u2010generation sequencing analysis. Subsequently, quantitative real\u2010time reverse\u2010transcription polymerase chain reaction was used to identify FGR placenta\u2010specific miRNAs whose level of expression was significantly decreased in FGR placenta (<jats:italic>n<\/jats:italic>\u2009=\u200945) compared with uncomplicated placenta (<jats:italic>n<\/jats:italic>\u2009=\u200950). FGR pregnancy\u2010associated, placenta\u2010specific microRNAs were identified in maternal plasma after delivery at significantly decreased concentrations, and their circulating levels in maternal plasma was compared between FGR pregnancies (<jats:italic>n<\/jats:italic>\u2009=\u200910) and uncomplicated pregnancies (<jats:italic>n<\/jats:italic>\u2009=\u200910).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of the ten placenta\u2010specific microRNAs that we identified, seven placenta\u2010specific microRNAs (hsa\u2010miR\u2010518b, hsa\u2010miR\u20101323, hsa\u2010miR\u2010516b, hsa\u2010miR\u2010515\u20105p, hsa\u2010miR\u2010520h, hsa\u2010miR\u2010519d, and hsa\u2010miR\u2010526b) from the chromosome 19 microRNA cluster were identified as FGR placenta\u2010specific microRNAs. Four FGR placenta\u2010specific microRNAs (hsa\u2010miR\u2010518b, hsa\u2010miR\u20101323, hsa\u2010miR\u2010520h, and hsa\u2010miR\u2010519d) were confirmed as FGR pregnancy\u2010associated, placenta\u2010specific miRNAs, but their circulating levels in maternal plasma showed no significant differences between FGR pregnancy and uncomplicated pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest that reduced expression in placenta of certain FGR placenta\u2010specific miRNAs is associated with FGR and that the discrepancy between expression in FGR placenta and their circulating levels in maternal plasma will be crucial to understanding how placenta\u2010specific microRNAs are released into the maternal circulation. \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.4045","type":"journal-article","created":{"date-parts":[[2013,1,28]],"date-time":"2013-01-28T04:25:05Z","timestamp":1359347105000},"page":"214-222","source":"Crossref","is-referenced-by-count":135,"title":["Characterization of placenta\u2010specific microRNAs in fetal growth restriction pregnancy"],"prefix":"10.1002","volume":"33","author":[{"given":"Ai","family":"Higashijima","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyonori","family":"Miura","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Mishima","sequence":"additional","affiliation":[{"name":"Human Genetics Nagasaki University Graduate 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