{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:18:44Z","timestamp":1791177524817,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,1,17]],"date-time":"2014-01-17T00:00:00Z","timestamp":1389916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to identify a set of predominantly placental (PP) mRNAs, which are associated with later\u2010developing twin\u2010to\u2010twin transfusion syndrome (TTTS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>First, out of 50 PP mRNAs we previously reported, we select target mRNAs that are ordinarily detectable in maternal plasma. Plasma concentrations of these PP mRNAs were measured in monochorionic diamniotic twin (MCDA\u2010T) pregnancies complicated by TTTS later (<jats:italic>n<\/jats:italic>\u2009=\u200911) and in uncomplicated MCDA\u2010T pregnancies (<jats:italic>n<\/jats:italic>\u2009=\u200917). Finally, the diagnostic values of the PP mRNAs in plasma were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>From 50 PP mRNAs, nine [human placental lactogen (<jats:italic>hPL<\/jats:italic>); pregnancy\u2010specific glycoproteins 2 (<jats:italic>PSG2<\/jats:italic>); human pregnancy\u2010specific glycoproteins 3 (<jats:italic>PSG3<\/jats:italic>); <jats:italic>syncytin<\/jats:italic>; <jats:italic>syncytin 2<\/jats:italic>; retinoic acid\u2010induced 14; A disintegrin and metalloproteinase domain\u2010containing protein 12 (<jats:italic>ADAM12<\/jats:italic>); chorionic glycoprotein hormones, alpha polypeptide; and chorionic glycoprotein hormones, and beta polypeptide] were selected as target mRNAs. Changes in six PP mRNAs [increased <jats:italic>hPL<\/jats:italic>, <jats:italic>PSG2<\/jats:italic>, and <jats:italic>PSG3<\/jats:italic> and decreased <jats:italic>syncytin<\/jats:italic>, <jats:italic>syncytin2<\/jats:italic>, and <jats:italic>ADAM12<\/jats:italic>] in maternal plasma were detected in MCDA\u2010T pregnant women who subsequently developed TTTS. Finally, mRNA signatures gave elevated AUCs (<jats:italic>hPL<\/jats:italic>\/<jats:italic>PSG2<\/jats:italic>: 0.8717; <jats:italic>hPL<\/jats:italic>\/<jats:italic>PSG3<\/jats:italic>: 0.8449; <jats:italic>hPL<\/jats:italic>\/<jats:italic>ADAM12<\/jats:italic>: 0.8396) compared with single <jats:italic>hPL<\/jats:italic> mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Quantitative aberration of plural cell\u2010free PP mRNAs in maternal plasma precedes the appearance of clinically apparent TTTS. This suggests that pathophysiological changes in the placenta are associated with morbid conditions of TTTS. \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.4307","type":"journal-article","created":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T05:38:37Z","timestamp":1387777117000},"page":"345-349","source":"Crossref","is-referenced-by-count":9,"title":["Predominantly placenta\u2010expressed mRNAs in maternal plasma as predictive markers for twin\u2013twin transfusion syndrome"],"prefix":"10.1002","volume":"34","author":[{"given":"Kiyonori","family":"Miura","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Nagasaki University Graduate School of Biomedical Sciences  Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai","family":"Higashijima","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Nagasaki University Graduate School of Biomedical Sciences 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