{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T08:30:08Z","timestamp":1723105808363},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,2,28]],"date-time":"2016-02-28T00:00:00Z","timestamp":1456617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Fetal endoscopic tracheal occlusion in congenital diaphragmatic hernia (CDH) may reduce pulmonary hypertension and ameliorate postnatal cardiac output. The effects of sustained early (ETO) and late (LTO) tracheal occlusion on left ventricular (LV) cells in the lamb model have not been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>CDH was created in lambs at 70\u2009days' gestation (term\u2009=\u2009145\u2009days). ETO (85\u2009days) or LTO (105\u2009days) was sustained till term. After cesarean section (140\u2009days) fetuses were euthanized and hearts harvested. LV myocardial cells were studied by histological and immunofluorescence (TGF\u2010beta 1, endothelin\u20101) assays in CDH, ETO, LTO, and the control group (two subjects per group). Small intramyocardial arteries were evaluated by traditional histology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LV myocardial histology in CDH and LTO was similar. ETO\u2010induced LV myocardial cell enlargement and increased endothelin\u20101 and TGF\u2010beta 1 staining; a weaker immunofluorescence signal was observed in LTO compared with ETO. Myocardial vascular wall thickness was greater in CDH than in controls. ETO was associated with a vascular wall thickness within the range of controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>With only two fetuses in each group, only an explorative evaluation was possible. The time point at which TO is performed seems to have an effect on cardiac morphology. Functional studies as well as confirmation in clinical samples are mandatory. \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.4789","type":"journal-article","created":{"date-parts":[[2016,2,6]],"date-time":"2016-02-06T07:04:40Z","timestamp":1454742280000},"page":"362-367","source":"Crossref","is-referenced-by-count":4,"title":["Myocardial effects of fetal endoscopic tracheal occlusion in lambs with CDH"],"prefix":"10.1002","volume":"36","author":[{"given":"Elisa","family":"Zambaiti","sequence":"first","affiliation":[{"name":"Pediatric Surgery Unit, Department of Clinical\u2010Surgical, Diagnostic and Pediatric Sciences University of Pavia  Pavia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossana","family":"Bussani","sequence":"additional","affiliation":[{"name":"Institute of Pathologic Anatomy University of Trieste  Trieste 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