{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:41:40Z","timestamp":1782351700257,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T00:00:00Z","timestamp":1464825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This work aimed to study the effect of uteroplacental circulation restriction on endothelial kidney damage in a fetal rabbit model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>New Zealand rabbits were subjected to 40% to 50% of uteroplacental artery ligation at day 25 of pregnancy. After 5\u2009days, surviving fetuses were harvested by cesarean section. The gene and protein expressions of selected enzymes associated with nitric oxide production and oxidative stress were analyzed in fetal kidney homogenates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The placenta weight (6.06\u2009\u00b1\u20090.27, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0319) and fetal body (19.90\u2009\u00b1\u20091.03, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) were significantly reduced in the uteroplacental circulation restriction group. The kidneys from restricted fetuses presented a mild vascular congestion and glomerular capillary congestion, without inflammation or hypertrophy. We found endothelial nitric oxide synthase phosphorylation inhibition (0.23\u2009\u00b1\u20090.13, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.012) and arginase\u20102 (0.29\u2009\u00b1\u20090.14, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.023) protein induction in fetal kidneys of the circulation restriction group. Finally, the kidneys from circulation\u2010restricted fetuses showed increased inducible nitric oxide synthase messenger RNA (mRNA) (2.68\u2009\u00b1\u20090.24, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) and reduced heme oxygenase\u20101 mRNA (23\u2009\u00b1\u20091.3, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.003), with increased reactive oxygen species (1.69\u2009\u00b1\u20090.09, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and nitrotyrosine protein (1.74\u2009\u00b1\u20090.28, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.003) levels, without changes in Nox mRNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We describe significant deregulation of vascular activity and oxidative damage in kidneys of fetal rabbits that have been exposed to restriction of the uterine circulation. \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.4829","type":"journal-article","created":{"date-parts":[[2016,4,25]],"date-time":"2016-04-25T06:06:40Z","timestamp":1461564400000},"page":"628-635","source":"Crossref","is-referenced-by-count":9,"title":["Nitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction"],"prefix":"10.1002","volume":"36","author":[{"given":"Horacio","family":"Figueroa","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology and Laboratory of Reproductive Biology, Faculty of Medicine Universidad de los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Cifuentes","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Integrative Physiology, Faculty of Medicine Universidad de los Andes  Santiago 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