{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:24:40Z","timestamp":1785194680535,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T00:00:00Z","timestamp":1488758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study was aimed to evaluate whether maternal dried blood spots could be a potential source for the noninvasive fetal <jats:italic>RHD<\/jats:italic> genotyping, serving as a combined one\u2010step test for both the First Trimester Screen and the fetal <jats:italic>RHD<\/jats:italic> genotyping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Both the maternal dried blood spots and the peripheral blood samples from 19 RhD\u2010negative pregnant women were obtained during the First Trimester Screen. DNA was extracted and sequential real\u2010time PCRs were performed to determine the fetal <jats:italic>RHD<\/jats:italic> genotypes. Fetal RhD serological types were obtained after delivery. This study was approved by the Institutional Review Board, and informed consents were obtained.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 19\/19 fetal <jats:italic>RHD<\/jats:italic> genotyping with maternal DBS were consistent with the follow\u2010up serological RhD test results after birth. Eleven were RhD positive, and eight were RhD negative (<jats:italic>RHD<\/jats:italic> deletion or <jats:italic>RHD\u2010CE\u2010D<\/jats:italic>\u2009=\u20096, <jats:italic>RHD<\/jats:italic> pseudogene\u2009=\u20091, <jats:italic>RHD<\/jats:italic>VI\u2009=\u20091). Sensitivity\u2009=\u2009100%, specificity\u2009=\u2009100%, positive predictive value\u2009=\u2009100%, negative predictive value\u2009=\u2009100%. A total of 18\/19 fetal gender were determined correctly with maternal DBS. One female fetus was falsely determined as male. Sensitivity\u2009=\u2009100%, specificity\u2009=\u200991.6%, positive predictive value\u2009=\u200987.5%, negative predictive value\u2009=\u2009100%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Maternal dried blood spots, with the benefits of flexible sample transportation and processing, could be utilized for the noninvasive prenatal fetal <jats:italic>RHD<\/jats:italic> genotyping and potentially be incorporated into the routine First Trimester Screen. Larger scale study is in progress to implement fetal <jats:italic>RHD<\/jats:italic> genotyping in routine prenatal care. \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.5006","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T03:48:06Z","timestamp":1484279286000},"page":"311-317","source":"Crossref","is-referenced-by-count":5,"title":["First trimester noninvasive fetal <i>RHD<\/i> genotyping using maternal dried blood spots"],"prefix":"10.1002","volume":"37","author":[{"given":"Yali","family":"Xiong","sequence":"first","affiliation":[{"name":"Fels Institute for Cancer Research and Molecular Biology Lewis Katz School of Medicine, Temple University  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey","family":"Jeronis","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences Lewis Katz School of Medicine, Temple University  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Fels Institute for Cancer Research and Molecular Biology Lewis Katz School of Medicine, Temple University  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan A.","family":"Liebermann","sequence":"additional","affiliation":[{"name":"Fels Institute for Cancer Research and Molecular Biology Lewis Katz School of Medicine, Temple University  Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ossie","family":"Geifman\u2010Holtzman","sequence":"additional","affiliation":[{"name":"Fels Institute for Cancer Research and Molecular Biology Lewis Katz School of Medicine, Temple University  Philadelphia PA USA"},{"name":"Department of Obstetrics and Gynecology College of Medicine, Drexel University  Philadelphia PA 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