{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:19:37Z","timestamp":1790518777648,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,16]],"date-time":"2017-03-16T00:00:00Z","timestamp":1489622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Prenatal Diagnosis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study is to investigate the diagnostic value of invasive prenatal diagnosis (PD) of congenital cytomegalovirus (CMV) infection from amniotic fluid (AF) and fetal blood (FB).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A retrospective study was conducted on 115 pregnancies with CMV primary infection. A total of 111 AF and 106 FB samples were investigated for various virological and non\u2010virological markers. Detailed ultrasound examinations were performed at time of PD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall sensitivity of CMV PCR in FB (75.6%; 95%CI 60\u201387) and AF (72.7%; 95%CI 57\u201385) was comparable. In women with amniocentesis &gt;8\u2009weeks between seroconversion and PD, we did not observe significant differences between amniocentesis performed \u226517\u2009+\u20090 (sensitivity 90.9%; 95%CI 71\u201399) and \u226520\u2009+\u20090 gestational weeks (sensitivity 90.0%; 95%CI 68\u201399). Virological markers in FB were higher in symptomatic compared with asymptomatic fetuses (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). No significant differences were observed for non\u2010virological markers. However, platelet counts &lt;120\u2009\u00d7\u200910e9\/L and beta\u20102 microglobulin values &gt;14\u2009mg\/L were more frequently found in fetuses with severe ultrasound abnormalities compared with fetuses with no or mild abnormalities (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Optimal timing of amniocentesis in women with primary infection in early gestation should be reevaluated in a prospective study. Analysis of FB markers may be beneficial in the individual management of pregnant women with confirmed congenital CMV infection. \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.5025","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T15:33:08Z","timestamp":1487259188000},"page":"389-398","source":"Crossref","is-referenced-by-count":76,"title":["Prenatal diagnosis of congenital cytomegalovirus infection in 115 cases: a 5 years' single center experience"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3218-074X","authenticated-orcid":false,"given":"M.","family":"Enders","sequence":"first","affiliation":[{"name":"Laboratory Prof. Gisela Enders &amp; Colleagues MVZ and Institute of Virology, Infectiology and Epidemiology e.V.  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