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We also explored the feasibility of obtaining trio biospecimens by using parental engagement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A convenience sample of women with discordant cfDNA results were recruited by their care providers. We provided shipping materials and instructions for biospecimen collection. Maternal, newborn, and placental samples were examined with droplet digital PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirteen of 15 women successfully had biospecimens obtained remotely. High\u2010quality DNA was extracted in 12 of 13 women. Presumed biologic etiologies for discordance were identified in 7 of 12 women: 3 cases from additional clinical review (male renal transplant, vanishing twin, and colon cancer) and 4 cases from additional laboratory investigation using droplet digital PCR (3 with confined placental mosaicism and 1 with true fetal mosaicism).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Understanding the biology behind cfDNA\u2010fetal karyotype discordancy is useful for follow\u2010up clinical care. Our study suggests that most cases could be resolved by using a trio biospecimen protocol and parental involvement. To improve accuracy, additional sequencing of biospecimens will be required.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pd.5260","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T00:40:57Z","timestamp":1523320857000},"page":"445-458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Biological explanations for discordant noninvasive prenatal test results: Preliminary data and lessons learned"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6077-0953","authenticated-orcid":false,"given":"Louise","family":"Wilkins\u2010Haug","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Brigham and Women's Hospital  Boston MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengsheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jackson Laboratory for Genomic Medicine  Farmington CT 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